論文 - 詳細
| RRC ID | 44224 |
|---|---|
| 著者 | Nitta H, Wada Y, Kawano Y, Murakami Y, Irie A, Taniguchi K, Kikuchi K, Yamada G, Suzuki K, Honda J, Wilson-Morifuji M, Araki N, Eto M, Baba H, Imamura T. |
| タイトル | Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a receptor (CD88). |
| ジャーナル | Clin Cancer Res |
| Abstract |
PURPOSE:The anaphylatoxin C5a is a chemoattractant that induces leukocyte migration via C5a receptor (C5aR). There is emerging evidence that C5a is generated in the cancer microenvironment. We therefore sought C5aR expression and a direct influence of the C5a-C5aR axis on cancer cells. EXPERIMENTAL DESIGN:C5aR expression was investigated in human cancer tissues and cell lines. Effects of C5a stimulation on cancer cells were studied by cytoskeletal rearrangement, time-lapse analysis, Matrigel chamber assay, and invasion in nude mouse in a comparison of C5aR-expressing cancer cells with control cells. RESULTS:C5aR was aberrantly expressed in various human cancers. Several cancer cell lines also expressed C5aR. C5a triggered cytoskeletal rearrangement and enhanced cell motility three-fold and invasiveness 13-fold of C5aR-expressing cancer cells. Such enhancement by C5a was not observed in control cells. Cancer cell invasion was still enhanced in the absence of C5a concentration gradient and even after the removal of C5a stimulation, suggesting that random cell locomotion plays an important role in C5a-triggered cancer cell invasion. C5a increased the release of matrix metalloproteinases (MMP) from cancer cells by two- to 11-fold, and inhibition of MMP activity abolished the C5a-enhancing effect on cancer cell invasion. Compared with control cells, C5aR-expressing cells spread 1.8-fold more broadly at implanted nude mouse skin sites only when stimulated with C5a. CONCLUSIONS:These results illustrate a novel activity of the C5a-C5aR axis that promotes cancer cell invasion through motility activation and MMP release. Targeting this signaling pathway may provide a useful therapeutic option for cancer treatment. |
| 巻・号 | 19(8) |
| ページ | 2004-13 |
| 公開日 | 2013-4-15 |
| DOI | 10.1158/1078-0432.CCR-12-1204 |
| PII | 1078-0432.CCR-12-1204 |
| PMID | 23287562 |
| MeSH | Aniline Compounds / pharmacology Animals Cell Line, Tumor Cell Movement / drug effects* Cell Movement / genetics Complement C5a / pharmacology* Cytoskeleton / drug effects Cytoskeleton / metabolism Gene Expression Regulation, Neoplastic HCT116 Cells Humans Immunoblotting Immunohistochemistry Matrix Metalloproteinases / metabolism Mice Mice, Inbred BALB C Mice, Nude Microscopy, Confocal Neoplasm Invasiveness Neoplasms / genetics Neoplasms / metabolism* Neoplasms / pathology Receptor, Anaphylatoxin C5a / antagonists & inhibitors Receptor, Anaphylatoxin C5a / genetics* Receptor, Anaphylatoxin C5a / metabolism* Reverse Transcriptase Polymerase Chain Reaction Tetrahydronaphthalenes / pharmacology Time-Lapse Imaging Transplantation, Heterologous |
| IF | 10.107 |
| 引用数 | 48 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | SSP-25(RCB1293) |