| RRC ID |
44226
|
| 著者 |
Miyatake K, Tsuji K, Yamaga M, Yamada J, Matsukura Y, Abula K, Sekiya I, Muneta T.
|
| タイトル |
Human YKL39 (chitinase 3-like protein 2), an osteoarthritis-associated gene, enhances proliferation and type II collagen expression in ATDC5 cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Human YKL39 (chitinase 3-like protein 2/CHI3L2) is a secreted 39kDa protein produced by articular chondrocytes and synoviocytes. Recent studies showed that hYKL-39 expression is increased in osteoarthritic articular chondrocytes suggesting the involvement of hYKL-39 in the progression of osteoarthritis (OA). However little is known regarding the molecular function of hYKL-39 in joint homeostasis. Sequence analyses indicated that hYKL-39 has significant identity with the human chitotorisidase family molecules, although it is considered that hYKL-39 has no enzymatic activity since it lacks putative chitinase catalytic motif. In this study, to examine the molecular function of hYKL-39 in chondrocytes, we overexpressed hYKL-39 in ATDC5 cells. Here we report that hYKL-39 enhances colony forming activity, cell proliferation, and type II collagen expression in these cells. These data suggest that hYKL-39 is a novel growth and differentiation factor involved in cartilage homeostasis.
|
| 巻・号 |
431(1)
|
| ページ |
52-7
|
| 公開日 |
2013-2-1
|
| DOI |
10.1016/j.bbrc.2012.12.094
|
| PII |
S0006-291X(12)02454-0
|
| PMID |
23291184
|
| MeSH |
Animals
Cell Line
Cell Proliferation*
Chitinases / genetics
Chitinases / physiology*
Chondrocytes / cytology
Chondrocytes / metabolism
Chondrocytes / physiology*
Collagen Type II / biosynthesis*
G1 Phase
Homeostasis
Humans
Mice
Osteoarthritis / genetics
S Phase
|
| IF |
2.985
|
| 引用数 |
9
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
