RRC ID 44239
Author Kudo TA, Kanetaka H, Shimizu Y, Abe T, Mori H, Mori K, Suzuki E, Takagi T, Izumi S.
Title Induction of neuritogenesis in PC12 cells by a pulsed electromagnetic field via MEK-ERK1/2 signaling.
Journal Cell Struct. Funct.
Abstract We examined the regulation of neuritogenesis by a pulsed electromagnetic field (PEMF) in rat PC12 pheochromocytoma cells, which can be induced to differentiate into neuron-like cells with elongated neurites by inducers such as nerve growth factor (NGF). Plated PC12 cells were exposed to a single PEMF (central magnetic flux density, 700 mT; frequency, 0.172 Hz) for up to 12 h per day and were then evaluated for extent of neuritogenesis or acetylcholine esterase (AChE) activity. To analyze the mechanism underlying the effect of the PEMF on the cells, its effects on intracellular signaling were examined using the ERK kinase (MEK) inhibitors PD098059 and U0126 (U0124 was used as a negative control for U0126). The number of neurite-bearing PC12 cells and AChE activity increased after PEMF exposure without the addition of other inducers of neuritogenesis. Additionally, PEMF exposure induced sustained activation of ERK1/2 in PC12 cells, but not in NR8383 rat alveolar macrophages. Furthermore, U0126 strongly inhibited PEMF-dependent ERK1/2 activation and neuritogenesis. The PEMF-dependent neuritogenesis was also suppressed by PD098059, but not U0124. These results suggest that PEMF stimulation independently induced neuritogenesis and that activation of MEK-ERK1/2 signaling was induced by a cell-type-dependent mechanism required for PEMF-dependent neuritogenesis in PC12 cells.
Volume 38(1)
Pages 15-20
Published 2013
DOI 10.1247/csf.12030
PMID 23318214
MeSH Animals Butadienes / pharmacology Cell Differentiation* / drug effects Cell Differentiation* / genetics Cell Differentiation* / radiation effects Electromagnetic Fields Flavonoids / pharmacology Humans MAP Kinase Signaling System / drug effects MAP Kinase Signaling System / radiation effects Macrophages, Alveolar / metabolism Macrophages, Alveolar / radiation effects Nerve Growth Factor* / drug effects Nerve Growth Factor* / metabolism Nerve Growth Factor* / radiation effects Neurites* / drug effects Neurites* / physiology Neurites* / radiation effects Nitriles / pharmacology PC12 Cells Rats
IF 3.5
Times Cited 7
Human and Animal Cells