RRC ID 44256
著者 Morigaki K, Mizutani K, Saito M, Okazaki T, Nakajima Y, Tatsu Y, Imaishi H.
タイトル Surface functionalization of a polymeric lipid bilayer for coupling a model biological membrane with molecules, cells, and microstructures.
ジャーナル Langmuir
Abstract We describe a stable and functional model biological membrane based on a polymerized lipid bilayer with a chemically modified surface. A polymerized lipid bilayer was formed from a mixture of two diacetylene-containing phospholipids, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) and 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine (DiynePE). DiynePC formed a stable bilayer structure, whereas the ethanolamine headgroup of DiynePE enabled functional molecules to be grafted onto the membrane surface. Copolymerization of DiynePC and DiynePE resulted in a robust bilayer. Functionalization of the polymeric bilayer provided a route to a robust and biomimetic surface that can be linked with biomolecules, cells, and three-dimensional (3D) microstructures. Biotin and peptides were grafted onto the polymeric bilayer for attaching streptavidin and cultured mammalian cells by molecular recognition, respectively. Nonspecific adsorption of proteins and cells on polymeric bilayers was minimum. DiynePE was also used to attach a microstructure made of an elastomer (polydimethylsiloxan: PDMS) onto the membrane, forming a confined aqueous solution between the two surfaces. The microcompartment enabled us to assay the activity of a membrane-bound enzyme (cyochrome P450). Natural (fluid) lipid bilayers were incorporated together with membrane-bound proteins by lithographically polymerizing DiynePC/DiynePE bilayers. The hybrid membrane of functionalized polymeric bilayers and fluid bilayers offers a novel platform for a wide range of biomedical applications including biosensor, bioassay, cell culture, and cell-based assay.
巻・号 29(8)
ページ 2722-30
公開日 2013-2-26
DOI 10.1021/la304747e
PMID 23347422
MeSH Adsorption Animals Biomimetic Materials / chemical synthesis Biomimetic Materials / chemistry* Cell Adhesion Fibroblasts / cytology* Lipid Bilayers / chemical synthesis Lipid Bilayers / chemistry* Mice Models, Molecular Molecular Structure NIH 3T3 Cells Polymerization Polymers / chemical synthesis Polymers / chemistry* Proteins / chemistry* Streptavidin / chemistry* Surface Properties
IF 3.557
引用数 10
WOS 分野 MATERIALS SCIENCE, MULTIDISCIPLINARY CHEMISTRY, MULTIDISCIPLINARY CHEMISTRY, PHYSICAL
リソース情報
ヒト・動物細胞 NIH3T3