RRC ID 44316
Author Tiwari A, Sharma Y, Hattori S, Terada D, Sharma AK, Turner AP, Kobayashi H.
Title Influence of poly(n-isopropylacrylamide)-CNT-polyaniline three-dimensional electrospun microfabric scaffolds on cell growth and viability.
Journal Biopolymers
Abstract This study investigates the effect on: (1) the bulk surface and (2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacrylamide)-CNT-polyaniline on growth and viability of cells. The poly(N-isopropylacrylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non-woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 μm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacrylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacrylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N-isopropylacrylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N-isopropylacrylamide). The results suggest that poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering.
Volume 99(5)
Pages 334-41
Published 2013-5-1
DOI 10.1002/bip.22170
PMID 23426576
MeSH Acrylic Resins / chemistry* Aniline Compounds / chemistry* Animals Cell Adhesion Cell Culture Techniques / methods Cell Proliferation* Cell Survival Collagen Drug Combinations Electrochemical Techniques / methods Fibroblasts / cytology L Cells Laminin Magnetic Resonance Spectroscopy Mice Microscopy, Electron, Scanning Polymers / chemistry* Proteoglycans Temperature Time Factors Tissue Engineering / methods* Tissue Scaffolds / chemistry*
IF 1.854
Times Cited 30
Human and Animal Cells