| RRC ID |
44317
|
| Author |
Thang NM, Kumasawa K, Tsutsui T, Nakamura H, Masaki H, Ono T, Kimura T.
|
| Title |
Overexpression of endogenous TIMP-2 increases the proliferation of BeWo choriocarcinoma cells through the MAPK-signaling pathway.
|
| Journal |
Reprod Sci
|
| Abstract |
Choriocarcinoma is a highly malignant form of trophoblastic tumor that is characterized by malignant placental tumors and rapid cell growth. In vivo and in vitro studies have demonstrated that tissue inhibitor of metalloproteinase 2 (TIMP-2) is present in choriocarcinoma. However, the role of TIMP-2 in cell proliferation in choriocarcinoma has not been investigated. Exogenous TIMP-2 is known to promote cell proliferation. During growth, cells are subjected to varied concentrations of TIMP-2, which depend on the amount of TIMP-2 produced by the cells themselves. Thus, the effect of gradually increasing endogenous TIMP-2 on the proliferation of choriocarcinoma cells needs to be examined. Proliferation of BeWo human choriocarcinoma cells was stimulated by transient transfection of a plasmid expressing TIMP-2. Overexpression of endogenous TIMP-2 also activated ERK1/2 and JNK1/2 of the MAPK-signaling pathway. Furthermore, inhibition of these proteins resulted in suppression of the cell proliferation-stimulating effect of TIMP-2. These results suggest that TIMP-2 plays an important role in tumor growth in the case of BeWo cells. Moreover, proliferation of BeWo cells due to TIMP-2 expression can be used as a model for fast-growing choriocarcinomas, and TIMP-2 could be used as a novel tumor marker of choriocarcinoma.
|
| Volume |
20(10)
|
| Pages |
1184-92
|
| Published |
2013-10-1
|
| DOI |
10.1177/1933719113477485
|
| PII |
1933719113477485
|
| PMID |
23427184
|
| MeSH |
Cell Proliferation*
Choriocarcinoma / enzymology*
Choriocarcinoma / pathology
Gene Expression Regulation, Neoplastic*
HEK293 Cells
Humans
MAP Kinase Signaling System / physiology*
Tissue Inhibitor of Metalloproteinase-2 / biosynthesis*
|
| IF |
2.616
|
| Times Cited |
8
|
|
WOS Category
|
OBSTETRICS & GYNECOLOGY
REPRODUCTIVE BIOLOGY
|
| Resource |
| Human and Animal Cells |
293T(RCB2202) |
