論文 - 詳細
| RRC ID | 44328 |
|---|---|
| 著者 | Maeda S, Maeda S, Ohno K, Kaji N, Hori M, Fujino Y, Tsujimoto H. |
| タイトル | Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes. |
| ジャーナル | Vet Immunol Immunopathol |
| Abstract |
Although the molecular basis of the allergenicity remains to be fully elucidated, the ability of allergens to elicit allergic responses is at least partly attributed to their proteolytic activity. Protease-activated receptor-2 (PAR-2) is a G protein-coupled receptor that is activated by site-specific proteolysis by serine proteases and is known to mediate inflammatory processes in various tissues. In this study, we investigated the effects of trypsin, a major serine protease, and a human PAR-2 agonist peptide (SLIGKV-NH2) on proinflammatory cytokine and chemokine gene expression in the canine keratinocyte cell line CPEK. The expression of PAR-2 mRNA and protein in CPEK cells was detected by RT-PCR and Western blotting, respectively. The localization of PAR-2 in CPEK was examined by immunofluorescence. The mRNA expression levels of proinflammatory cytokines and chemokines were quantified by real-time RT-PCR. The free intracellular Ca(2+) concentration was measured using the Ca(2+)-sensitive fluorescent dye. CPEK cells constitutively expressed PAR-2 mRNA and protein. Stimulation of CPEK cells with trypsin induced significant upregulation of the mRNA expression levels of tumor necrosis factor alpha (TNF-α, P<0.05), granulocyte-macrophage colony-stimulating factor (GM-CSF, P<0.01), thymus and activation regulated chemokine (TARC/CCL17, P<0.01), and interleukin 8 (IL-8/CXCL8, P<0.01). Similarly, the PAR-2 agonist peptide increased the mRNA expression levels of TNF-α (P<0.05), GM-CSF (P<0.05), TARC/CCL17 (P<0.05), and IL-8/CXCL8 (P<0.05) in CPEK cells. Both trypsin and the PAR-2 agonist peptide increased the intracellular Ca(2+) concentration and PAR-2 internalization. These results suggest that PAR-2 activation can augment inflammatory cytokine and chemokine expression in canine keratinocytes, and it may initiate allergic inflammation through the proteolytic activity of allergens in canine atopic dermatitis. |
| 巻・号 | 153(1-2) |
| ページ | 17-25 |
| 公開日 | 2013-5-15 |
| DOI | 10.1016/j.vetimm.2013.01.018 |
| PII | S0165-2427(13)00065-2 |
| PMID | 23465358 |
| MeSH | Animals Calcium / metabolism Chemokines / genetics Cross Reactions Cytokines / genetics Dermatitis, Atopic / etiology Dogs HEK293 Cells Humans Keratinocytes / immunology* RNA, Messenger / analysis Receptor, PAR-2 / agonists Receptor, PAR-2 / physiology* Trypsin / pharmacology |
| IF | 1.713 |
| 引用数 | 11 |
| WOS 分野 | VETERINARY SCIENCES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |