RRC ID 44332
著者 Otsuki K, Imaizumi M, Nomoto Y, Wada I, Miyake M, Sugino T, Omori K.
タイトル Potential for respiratory epithelium regeneration from induced pluripotent stem cells.
ジャーナル Ann Otol Rhinol Laryngol
Abstract OBJECTIVES:In cases of laryngeal inflammatory lesions and tracheal invasion of a malignant tumor, autologous tissue implantation techniques using skin or cartilage are often applied. However, these techniques are both invasive and unstable. The purpose of this study was to evaluate the potential use of induced pluripotent stem (iPS) cells in the regeneration of respiratory epithelium.
METHODS:We seeded iPS cells on low-attachment plates in serum-free media to generate embryoid bodies (EBs). After a 3-day culture, the EBs were transferred to a gelatin-coated dish supplemented with activin A alone or with basic fibroblast growth factor (induction groups). As a control, EBs were cultured without these growth factors (control group). Cultured tissues from all groups were histologically examined for 2 weeks.
RESULTS:In the induction groups, the presence of respiratory epithelium-like tissue was observed with hematoxylin and eosin staining after 14 days of culture.
CONCLUSIONS:This study demonstrated the potential use of iPS cells in regeneration of the respiratory epithelium.
巻・号 122(1)
ページ 25-32
公開日 2013-1-1
DOI 10.1177/000348941312200106
PMID 23472313
MeSH Cell Differentiation Cells, Cultured Culture Media, Serum-Free Guided Tissue Regeneration / methods* Humans Induced Pluripotent Stem Cells* Respiratory Mucosa / cytology Respiratory Mucosa / physiology*
IF 1.284
引用数 4
WOS 分野 OTORHINOLARYNGOLOGY
リソース情報
ヒト・動物細胞