RRC ID 44341
Author Wong CP, Kaneda T, Hadi AH, Morita H.
Title Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus.
Journal J Nat Med
Abstract The Meliaceae family of plants has been shown to contain a vast number of compounds with the potential to be developed for medicinal purposes. We have previously reported the isolation of limonoids from a plant in the Meliaceae family named Chisocheton ceramicus. Ceramicine B was identified as an active compound in inhibiting lipid droplets accumulation (LDA) in the mouse preadipocyte cell line MC3T3-G2/PA6. The presence of ceramicine B was found to inhibit the expression of glucose transporter type 4, lipoprotein lipase, and 11-beta hydroxysteroid dehydrogenase mRNA, and also adipogenic master regulator, peroxisome proliferator-activated receptor-γ, and CCAAT-enhancer-binding protein-α (C/EBPα) mRNA. However, for early adipogenic regulators, such as C/EBPβ and C/EBPδ, and intermediary adipogenic regulators, Krüppel-like factors were unaffected. Western blot analysis showed that ceramicine B was found to inhibit the phosphorylation of Forkhead box O1 (Foxo1), a key process in the insulin signaling pathway. This suggested that the mechanism of anti-LDA activity of ceramicine B was partly via the inhibition of Foxo1 phosphorylation.
Volume 68(1)
Pages 22-30
Published 2014-1-1
DOI 10.1007/s11418-013-0755-2
PMID 23494817
MeSH 11-beta-Hydroxysteroid Dehydrogenases / genetics 11-beta-Hydroxysteroid Dehydrogenases / metabolism 3T3 Cells Adipocytes / drug effects* Adipocytes / metabolism Adipogenesis / drug effects* Animals Anti-Obesity Agents / isolation & purification Anti-Obesity Agents / pharmacology* CCAAT-Enhancer-Binding Proteins / genetics CCAAT-Enhancer-Binding Proteins / metabolism Dose-Response Relationship, Drug Forkhead Box Protein O1 Forkhead Transcription Factors / metabolism Glucose Transporter Type 4 / genetics Glucose Transporter Type 4 / metabolism Kruppel-Like Transcription Factors / metabolism Limonins / isolation & purification Limonins / pharmacology* Lipoprotein Lipase / genetics Lipoprotein Lipase / metabolism Meliaceae* / chemistry Mice PPAR gamma / genetics PPAR gamma / metabolism Phosphorylation Phytotherapy Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plants, Medicinal RNA, Messenger / metabolism Signal Transduction / drug effects
IF 2.055
Times Cited 14
Human and Animal Cells MC3T3-G2/PA6(RCB1127)