論文 - 詳細
| RRC ID | 44346 |
|---|---|
| 著者 | Liao X, Leung KN. |
| タイトル | Tryptanthrin induces growth inhibition and neuronal differentiation in the human neuroblastoma LA-N-1 cells. |
| ジャーナル | Chem Biol Interact |
| Abstract |
Neuroblastoma is one of the most common extracranial solid cancers found in young children. The prognosis of neuroblastoma patients in advanced stages having N-myc amplification remains poor despite intensive multimodal therapy. Agents that trigger neuroblastoma cells to undergo cellular differentiation and thereby stop proliferation have attracted considerable interest as an alternative therapy. Tryptanthrin (12-dihydro-6,12-dioxoindolo-(2,1-b)-quinazoline) is a weakly basic alkaloid isolated from the dried roots of medicinal indigo plants known as Banlangen. It has been shown to possess various biological activities, such as anti-microbial, anti-inflammatory and anti-tumor activities. However, its effects and mechanism(s) of action on human neuroblastoma cells remain poorly understood. Therefore, the objective of this study is to investigate the effects of tryptanthrin on the growth and differentiation of human neuroblastoma LA-N-1 cells with N-myc amplification. Our results show that tryptanthrin inhibited the growth of the human neuroblastoma cells in a dose- and time-dependent manner. Mechanistic studies indicated that tryptanthrin induced cell cycle arrest of the human neuroblastoma LA-N-1 cells at the G0/G1 phase. Tryptanthrin also induced neuronal differentiation of LA-N-1 cells, as assessed by morphological criteria, enhancement of acetylcholine esterase activity and up-regulation of various differentiation markers. Moreover, tryptanthrin treatment led to the significant reduction of N-myc expression in LA-N-1 cells while siRNA directed against N-myc induced morphological differentiation of LA-N-1 cells. These results, when taken together, suggest that tryptanthrin suppressed the growth and induced neuronal differentiation in the human neuroblastoma LA-N-1 cells and might be exploited as a potential therapeutic candidate for the treatment of high-risk neuroblastomas with N-myc-amplification. |
| 巻・号 | 203(2) |
| ページ | 512-21 |
| 公開日 | 2013-4-25 |
| DOI | 10.1016/j.cbi.2013.03.001 |
| PII | S0009-2797(13)00042-2 |
| PMID | 23500671 |
| MeSH | Antineoplastic Agents / pharmacology* Biomarkers, Tumor / metabolism Cell Differentiation / drug effects* Cell Line, Tumor Cell Proliferation / drug effects Down-Regulation / drug effects G1 Phase Cell Cycle Checkpoints / drug effects Gene Expression Regulation, Neoplastic / drug effects Humans Neoplasm Invasiveness Neuroblastoma / pathology* Neurons / drug effects* Neurons / metabolism Neurons / pathology* Proto-Oncogene Proteins c-myc / genetics Quinazolines / pharmacology* Resting Phase, Cell Cycle / drug effects |
| IF | 3.723 |
| 引用数 | 13 |
| WOS 分野 | PHARMACOLOGY & PHARMACY TOXICOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LA-N-1(RCB0483) |