| RRC ID |
44376
|
| 著者 |
Komohara Y, Niino D, Saito Y, Ohnishi K, Horlad H, Ohshima K, Takeya M.
|
| タイトル |
Clinical significance of CD163⁺ tumor-associated macrophages in patients with adult T-cell leukemia/lymphoma.
|
| ジャーナル |
Cancer Sci
|
| Abstract |
In several malignant tumors including lymphoma, macrophages that infiltrate tumor tissues are called tumor-associated macrophages (TAMs). We discovered that TAMs, especially the CD163⁺ alternatively activated phenotype (M2), were closely involved with progression of adult T-cell leukemia/lymphoma (ATLL). We used CD68 (a pan-macrophage marker) and CD163 (an M2 marker) to immunostain 58 ATLL samples. Statistical analyses showed that a high number of CD68⁺ TAMs and an increased percentage of CD163⁺ cells among the TAMs were associated with a worse clinical prognosis; multivariate analysis indicated that the percentage of CD163⁺ cells was an independent prognostic factor. We also carried out in vitro coculture experiments with ATLL cell lines (ATN-1 and TL-Mor) and monocyte-derived macrophages and found that direct coculture with M2 macrophages significantly increased BrdU incorporation into ATLL cell lines. A cytokine array analysis showed that macrophage-derived soluble factors including C5a, tumor necrosis factor-α, growth-related oncogene-α, CCL1/I-309, and interleukin-6 stimulated ATLL cell lines. CD163 expression in macrophages was strongly induced by direct contact with ATN-1 cells, and downregulation of CD163 in macrophages significantly suppressed growth of cocultured ATN-1 cells. These results suggest that interaction between M2 macrophages and lymphoma cells may be an appropriate target in treatment of patients with ATLL.
|
| 巻・号 |
104(7)
|
| ページ |
945-51
|
| 公開日 |
2013-7-1
|
| DOI |
10.1111/cas.12167
|
| PMID |
23557330
|
| PMC |
PMC7657152
|
| MeSH |
Aged
Antigens, CD / immunology
Antigens, CD / metabolism*
Antigens, Differentiation, Myelomonocytic / immunology
Antigens, Differentiation, Myelomonocytic / metabolism*
Cell Line, Tumor
Chemokine CCL1 / metabolism
Chemokine CXCL1 / metabolism
Complement C5a / immunology
Complement C5a / metabolism
Disease Progression
Female
Humans
Interleukin-6 / metabolism
Leukemia-Lymphoma, Adult T-Cell / immunology
Leukemia-Lymphoma, Adult T-Cell / metabolism*
Leukemia-Lymphoma, Adult T-Cell / pathology*
Macrophages / immunology
Macrophages / metabolism*
Male
Prognosis
Receptors, Cell Surface / immunology
Receptors, Cell Surface / metabolism*
Tumor Necrosis Factor-alpha / metabolism
|
| IF |
4.966
|
| 引用数 |
60
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ATN-1(RCB1440)
TL-Mor(RCB1881) |
