論文 - 詳細
| RRC ID | 44382 |
|---|---|
| 著者 | Hirajima S, Komatsu S, Ichikawa D, Takeshita H, Konishi H, Shiozaki A, Morimura R, Tsujiura M, Nagata H, Kawaguchi T, Arita T, Kubota T, Fujiwara H, Okamoto K, Otsuji E. |
| タイトル | Clinical impact of circulating miR-18a in plasma of patients with oesophageal squamous cell carcinoma. |
| ジャーナル | Br J Cancer |
| Abstract |
BACKGROUND:Several recent studies demonstrated that microRNAs are stably detectable in plasma/serum. We tested whether miR-18a, which is located in the miR-17-92 cluster and reported to be highly expressed in tissues of oesophageal squamous cell carcinoma (ESCC), served as a plasma biomarker in patients with ESCC. METHODS:This study was divided into three steps: (1) confirmation of higher miR-18a levels in primary ESCC tissues and cell lines than normal ESCC tissues and a human fibroblast cell line. (2) Evaluation of the plasma miR-18a assay using quantitative RT-PCR by comparing results from 106 consecutive patients with ESCC and 54 healthy volunteers. (3) Evaluation of the assay for monitoring tumour dynamics in patients with ESCC. RESULTS:(1) Expression of miR-18a was significantly higher in ESCC tissues (P=0.0020) and ESCC cell lines (P=0.0121) than normal tissues and fibroblasts. (2) Plasma concentrations of miR-18a were significantly higher in ESCC patients than healthy volunteers (P<0.0001; ESCC patients vs healthy volunteers (mean±s.d.): 11.77±13.45 vs 0.73±0.54 amol μl(-1)). The value of the area under the receiver-operating characteristic (ROC) curve (AUC) was 0.9449. Furthermore, the ROC curves to detect early ESCC such as pTis-1 and pStage0-I showed AUCs of 0.9479 and 0.9642, respectively. (3) Plasma levels of miR-18a were significantly lower in postoperative samples than preoperative samples (P=0.0076). CONCLUSION:Plasma miR-18a may be a very useful biomarker for cancer detection and the monitoring of tumour dynamics in patients with ESCC. |
| 巻・号 | 108(9) |
| ページ | 1822-9 |
| 公開日 | 2013-5-14 |
| DOI | 10.1038/bjc.2013.148 |
| PII | bjc2013148 |
| PMID | 23579215 |
| PMC | PMC3658511 |
| MeSH | Aged Biomarkers, Tumor / blood* Biomarkers, Tumor / genetics Carcinoma, Squamous Cell / blood* Carcinoma, Squamous Cell / diagnosis Carcinoma, Squamous Cell / genetics Cell Line, Tumor Esophageal Neoplasms / blood* Esophageal Neoplasms / diagnosis Esophageal Neoplasms / genetics Humans MicroRNAs / biosynthesis MicroRNAs / blood* MicroRNAs / genetics Prognosis ROC Curve |
| IF | 5.791 |
| 引用数 | 72 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | TE-9(RCB1988) WI-38 |