論文 - 詳細
| RRC ID | 44388 |
|---|---|
| 著者 | Matsui T, Motoki Y, Yoshida Y. |
| タイトル | Hypothermia reduces toll-like receptor 3-activated microglial interferon-β and nitric oxide production. |
| ジャーナル | Mediators Inflamm |
| Abstract |
Therapeutic hypothermia protects neurons after injury to the central nervous system (CNS). Microglia express toll-like receptors (TLRs) that play significant roles in the pathogenesis of sterile CNS injury. To elucidate the possible mechanisms involved in the neuroprotective effect of therapeutic hypothermia, we examined the effects of hypothermic culture on TLR3-activated microglial release of interferon (IFN)- β and nitric oxide (NO), which are known to be associated with neuronal cell death. When rat or mouse microglia were cultured under conditions of hypothermia (33°C) and normothermia (37°C) with a TLR3 agonist, polyinosinic-polycytidylic acid, the production of IFN- β and NO in TLR3-activated microglia at 48 h was decreased by hypothermia compared with that by normothermia. In addition, exposure to recombinant IFN- β and sodium nitroprusside, an NO donor, caused death of rat neuronal pheochromocytoma PC12 cells in a concentration-dependent manner after 24 h. Taken together, these results suggest that the attenuation of microglial production of IFN- β and NO by therapeutic hypothermia leads to the inhibition of neuronal cell death. |
| 巻・号 | 2013 |
| ページ | 436263 |
| 公開日 | 2013-1-1 |
| DOI | 10.1155/2013/436263 |
| PMID | 23589665 |
| PMC | PMC3621171 |
| MeSH | Animals Cells, Cultured Hypothermia / metabolism* Interferon-beta / metabolism* Mice Microglia / drug effects Microglia / metabolism* Nitric Oxide / metabolism* Nitric Oxide Donors / pharmacology PC12 Cells Poly I-C / pharmacology Rats Rats, Wistar Temperature Toll-Like Receptor 3 / antagonists & inhibitors Toll-Like Receptor 3 / metabolism* |
| IF | 3.758 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |