論文 - 詳細
| RRC ID | 44400 |
|---|---|
| 著者 | Yin XF, Chen J, Mao W, Wang YH, Chen MH. |
| タイトル | Downregulation of aryl hydrocarbon receptor expression decreases gastric cancer cell growth and invasion. |
| ジャーナル | Oncol Rep |
| Abstract |
Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor associated with tumor initiation and progression. AhR expression is significantly increased in gastric cancer tissues and gastric cancer cell lines; however, the relationship between AhR and gastric cancer is still unclear. In the present study, we explored the effects of the inhibition of AhR expression by RNA interference on the biological behavior of gastric cancer cells (MKN45 and SGC7901), and elucidated the specific mechanisms of AhR action in the development of gastric cancer. Results showed that small interfering RNA (siRNA) against AhR effectively inhibited the expression of AhR, and decreased the expression of cytochrome P450 (CYP)1A1 and CYP1B1, which are classic target genes of the AhR pathway. Compared to the negative control group, AhR-siRNA-transfected cells showed decreased cellular growth, delayed G1-S cell cycle progression and increased apoptosis rate. Furthermore, inhibition of AhR expression by siRNA in SGC7901 cells led to decreased cell migratory and invasive ability, accompanied by downregulation of expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9. Our results, therefore, suggest that AhR promotes the growth and invasiveness of gastric cancer cells and AhR may serve as a promising therapeutic target for gastric cancer. |
| 巻・号 | 30(1) |
| ページ | 364-70 |
| 公開日 | 2013-7-1 |
| DOI | 10.3892/or.2013.2410 |
| PMID | 23604401 |
| MeSH | Aryl Hydrocarbon Hydroxylases / biosynthesis Cell Cycle / genetics Cell Line, Tumor Cell Movement / genetics Cell Proliferation Cytochrome P-450 CYP1A1 / biosynthesis Cytochrome P-450 CYP1B1 Down-Regulation Humans Matrix Metalloproteinase 2 / biosynthesis Matrix Metalloproteinase 9 / biosynthesis Neoplasm Invasiveness / genetics RNA Interference RNA, Small Interfering Receptors, Aryl Hydrocarbon / biosynthesis Receptors, Aryl Hydrocarbon / genetics* Receptors, Aryl Hydrocarbon / metabolism* Stomach Neoplasms / genetics* |
| IF | 3.417 |
| 引用数 | 13 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) |