| RRC ID |
44406
|
| 著者 |
Komori R, Kobayashi T, Matsuo H, Kino K, Miyazawa H.
|
| タイトル |
Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.
|
| ジャーナル |
PLoS One
|
| Abstract |
κ-Casein (CSN3) is known to play an essential role in controlling the stability of the milk micelles. We found that the expression of Csn3 was induced by all-trans retinoic acid (ATRA) during neural differentiation in P19 embryonal carcinoma cells from our study using DNA microarray. In this paper, we describe the detailed time course of Csn3 expression and the induction mechanism of Csn3 transcription activation in this process. The Csn3 expression was induced rapidly and transiently within 24 h of ATRA treatment. Retinoic acid receptor (RAR)-specific agonists were used in expression analysis to identify the RAR subtype involved upregulation of Csn3; a RARα-specific agonist mimicked the effects of ATRA on induction of Csn3 expression. Therefore, RARα may be the RAR subtype mediating the effects of ATRA on the induction of Csn3 gene transcription in this differentiation-promoting process of P19 cells. We found that the promoter region of Csn3 contained a typical consensus retinoic acid response element (RARE), and this RARE was necessary for ATRA-dependent transcriptional regulation. We confirmed that RARα bound to this RARE sequence in P19 cells. These findings indicated that the Csn3 expression is upregulated via ATRA-bound RARα and binding of this receptor to the RARE in the Csn3 promoter region. This will certainly serve as a first step forward unraveling the mysteries of induction of Csn3 in the process of neural differentiation.
|
| 巻・号 |
8(4)
|
| ページ |
e61938
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0061938
|
| PII |
PONE-D-12-31823
|
| PMID |
23613978
|
| PMC |
PMC3629135
|
| MeSH |
Animals
Base Sequence
COP9 Signalosome Complex
Cell Differentiation / drug effects
Cell Differentiation / genetics*
Cell Line
Embryo, Mammalian / cytology
Embryo, Mammalian / drug effects
Embryo, Mammalian / metabolism
Gene Expression Regulation / drug effects*
Mice
Molecular Sequence Data
Neurons / cytology*
Neurons / drug effects
Neurons / metabolism*
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Nucleotide Motifs / genetics
Protein Binding / drug effects
Protein Binding / genetics
Protein Kinases / genetics*
Protein Kinases / metabolism
Proto-Oncogene Proteins
Receptors, Retinoic Acid / metabolism
Response Elements / genetics
Retinoic Acid Receptor alpha
Transcription, Genetic / drug effects
Tretinoin / pharmacology*
|
| IF |
2.74
|
| 引用数 |
11
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
P19C6 |
