| RRC ID |
44434
|
| 著者 |
Kono H, Nakamura M, Ohtsuka T, Nagayoshi Y, Mori Y, Takahata S, Aishima S, Tanaka M.
|
| タイトル |
High expression of microRNA-155 is associated with the aggressive malignant behavior of gallbladder carcinoma.
|
| ジャーナル |
Oncol Rep
|
| Abstract |
The prognosis of gallbladder cancer (GBC) remains poor despite recent advances in diagnostics and therapeutic strategies. Although the role of microRNAs (miRs) in GBC have not been well documented, miR-155 is known to be associated with inflammation-associated carcinogenesis in various types of cancers. The aim of this study was to investigate the clinical significance of miR-155 expression and the biological functions of miR-155 in GBC. The expression levels of miR-155 in surgically resected GBCs and gallbladders with pancreaticobiliary maljunction (PBM) were assessed by quantitative reverse transcription-polymerase chain reaction. The relationship between the expression levels of miR-155 and clinicopathological features of GBCs was analyzed. Human GBC cell lines were transfected with miR-155 inhibitors or mimics, and the effects on proliferation and invasion were assessed. miR-155 was significantly overexpressed in GBCs when compared with that in gallbladders with PBM (p=0.007) and normal gallbladders (p=0.04). The high expression level of miR-155 in GBCs was significantly associated with the presence of lymph node metastasis (p=0.01) and a poor prognosis (p=0.02). In vitro assays showed that aberrant expression of miR-155 significantly enhanced GBC cell proliferation and invasion. In conclusion, high miR-155 expression correlates with the aggressive behavior of GBCs, and miR-155 may become a prognostic marker and therapeutic target for GBC.
|
| 巻・号 |
30(1)
|
| ページ |
17-24
|
| 公開日 |
2013-7-1
|
| DOI |
10.3892/or.2013.2443
|
| PMID |
23660842
|
| PMC |
PMC3729208
|
| MeSH |
Adult
Aged
Biomarkers, Tumor / genetics
Cell Line, Tumor
Cell Proliferation
Female
Gallbladder / cytology
Gallbladder Neoplasms / genetics*
Gene Expression
Gene Expression Regulation, Neoplastic
Humans
Inflammation / genetics
Lymphatic Metastasis / genetics*
Male
MicroRNAs / biosynthesis
MicroRNAs / genetics*
Middle Aged
Neoplasm Invasiveness / genetics*
Prognosis
|
| IF |
3.417
|
| 引用数 |
38
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
G-415(RCB2640) |
