論文 - 詳細
| RRC ID | 44464 |
|---|---|
| 著者 | Kawai R, Ozeki N, Yamaguchi H, Tanaka T, Nakata K, Mogi M, Nakamura H. |
| タイトル | Mouse ES cells have a potential to differentiate into odontoblast-like cells using hanging drop method. |
| ジャーナル | Oral Dis |
| Abstract |
OBJECTIVES:We examined whether mouse embryonic stem (ES) cells can differentiate into odontoblast-like cells without epithelial-mesenchymal interaction. MATERIALS AND METHODS:Cells were cultured by the 'hanging drop' method using a collagen type-I scaffold (CS) combined with bone morphogenetic protein (BMP)-4 (CS/BMP-4). Expression of odontoblast-related mRNA and protein, and cell proliferation were performed by reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence staining and WST-1 assay, respectively. RESULTS:Cells potently expressed odontoblast-related cell marker mRNAs following induction of odontoblastic differentiation. Dentin sialophosphoprotein, a marker of mature odontoblasts, was strongly expressed in differentiated ES cells. The cells also acquired an odontoblast-like functional phenotype, as evidenced by the appearance of alkaline phosphatase activity and calcification. The cell-surface expression of α2, α6, αV and αVβ3 integrin proteins was rapidly upregulated in differentiated cells. Finally, anti-α2 integrin antibody suppressed the expression of odontoblastic markers in cells grown using this culture system, suggesting that α2 integrin expression in ES cells triggers their differentiation into odontoblast-like cells. CONCLUSIONS:Mouse ES cells cultured by the 'hanging drop' method are able to differentiate into cells with odontoblast-specific physiological functions and cell-surface integrin protein expression. |
| 巻・号 | 20(4) |
| ページ | 395-403 |
| 公開日 | 2014-5-1 |
| DOI | 10.1111/odi.12134 |
| PMID | 23731055 |
| MeSH | Animals Cell Differentiation* Cells, Cultured Cytological Techniques / methods Embryonic Stem Cells / cytology* Mice Odontoblasts / cytology* |
| IF | 2.613 |
| 引用数 | 15 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| リソース情報 | |
| ヒト・動物細胞 | B6G-2(AES0003) |