論文 - 詳細
| RRC ID | 44473 |
|---|---|
| 著者 | Kim Y, Kelly OJ, Ilich JZ. |
| タイトル | Synergism of α-linolenic acid, conjugated linoleic acid and calcium in decreasing adipocyte and increasing osteoblast cell growth. |
| ジャーナル | Lipids |
| Abstract |
Whole fat milk and dairy products (although providing more energy compared to low- or non-fat products), are good sources of α-linolenic acid (ALA), conjugated linoleic acid (CLA) and calcium, which may be favorable in modulating bone and adipose tissue metabolism. We examined individual and/or synergistic effects of ALA, CLA and calcium (at levels similar to those in whole milk/dairy products) in regulating bone and adipose cell growth. ST2 stromal, MC3T3-L1 adipocyte-like and MC3T3-E1 osteoblast-like cells were treated with: (a) linoleic acid (LNA):ALA ratios = 1-5:1; (b) individual/combined 80-90 % c9, t11 (9,11) and 5-10 % t10, c12 (10,12) CLA isomers; (c) 0.5-3.0 mM calcium; (d) combinations of (a), (b), (c); and (e) control. Local mediators, including eicosanoids and growth factors, were measured. (a) The optimal effect was found at the 4:1 LNA:ALA ratio where insulin-like growth factor-1 (IGF-1) and IGF binding protein-3 (IGFBP-3) production was the lowest in MC3T3-L1 cells. (b) All CLA isomer blends decreased MC3T3-L1 and increased MC3T3-E1 cell differentiation. (c) 1.5-2.5 mM calcium increased ST2 and MC3T3-E1, and decreased MC3T3-L1 cell proliferation. (d) Combination of 4:1 LNA:ALA + 90:10 % CLA + 2.0 mM calcium lowered MC3T3-L1 and increased MC3T3-E1 cell differentiation. Overall, the optimal LNA:ALA ratio to enhance osteoblastogenesis and inhibit adipogenesis was 4:1. This effect was enhanced by 90:10 % CLA + 2.0 mM calcium, indicating possible synergism of these dietary factors in promoting osteoblast and inhibiting adipocyte differentiation in cell cultures. |
| 巻・号 | 48(8) |
| ページ | 787-802 |
| 公開日 | 2013-8-1 |
| DOI | 10.1007/s11745-013-3803-5 |
| PMID | 23757205 |
| MeSH | Adipocytes / drug effects* Adipocytes / metabolism Animals Calcium / metabolism Calcium / pharmacology* Cell Differentiation / drug effects Cell Line / drug effects Cell Proliferation / drug effects Drug Synergism Insulin-Like Growth Factor Binding Protein 3 / metabolism Insulin-Like Growth Factor I / metabolism Linoleic Acids, Conjugated / pharmacology* Mice Osteoblasts / drug effects* Osteoblasts / metabolism alpha-Linolenic Acid / pharmacology* |
| IF | 1.919 |
| 引用数 | 13 |
| WOS 分野 | NUTRITION & DIETETICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |