RRC ID 44475
Author Kim IS, Kim MJ, Shin DH, Son KH, Park HY, Lee JS.
Title Arazyme inhibits cytokine expression and upregulates skin barrier protein expression.
Journal Mol Med Rep
Abstract In the present study, the inhibitory effect of arazyme on allergic inflammation was investigated by evaluating the alteration of cytokine production and expression of skin barrier proteins in immune and HaCaT human keratinocyte cells. THP‑1 human monocytic and EoL‑1 human eosinophilic cells were treated with Dermatophagoides pteronissinus extract (DpE). Monocyte chemotactic protein‑1 (MCP‑1)/CCL2, interleukin (IL)‑6 and IL‑8 increased following DpE treatment and arazyme significantly blocked the increase of MCP‑1, IL‑6 and IL‑8 expression in cell types. Secretion of MCP‑1, IL‑6 and IL‑8 induced by lipopolysaccharide in THP‑1 cells was also inhibited by arazyme treatment. Arazyme inhibited the secretion of IL‑6 and IL‑8 due to phorbol 12‑myristate 13‑acetate and calcium ionophores in human mast cells. Arazyme blocked the secretion of thymus and activation‑regulated chemokine (TARC)/CCL17, MCP‑1, IL‑6 and IL‑8 due to tumor necrosis factor‑α (TNF‑α) and interferon‑γ (IFN‑γ) in HaCaT cells. TNF‑α and IFN‑γ suppressed the expression of skin barrier proteins, including filaggrin, involucrin and loricrin. By contrast, arazyme increased the expression of filaggrin, involucrin and loricrin. These results may contribute to the development of a therapeutic drug for the treatment of allergic diseases, including atopic dermatitis.
Volume 8(2)
Pages 551-6
Published 2013-8
DOI 10.3892/mmr.2013.1520
PMID 23764888
MeSH Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Line Chemokine CCL2 / biosynthesis Cytokines / genetics Cytokines / metabolism* Enterobacteriaceae / metabolism Eosinophils / drug effects Eosinophils / metabolism Eosinophils / pathology Gene Expression Regulation / drug effects Humans Interleukin-8 / biosynthesis Intermediate Filament Proteins / metabolism Keratinocytes / drug effects Keratinocytes / metabolism Membrane Proteins / metabolism Metalloproteases / isolation & purification Metalloproteases / pharmacology* Monocytes / drug effects Monocytes / immunology Monocytes / metabolism Protein Precursors / metabolism Skin / drug effects* Skin / immunology Skin / metabolism* Up-Regulation
IF 1.851
Times Cited 3
Human and Animal Cells