RRC ID 44475
著者 Kim IS, Kim MJ, Shin DH, Son KH, Park HY, Lee JS.
タイトル Arazyme inhibits cytokine expression and upregulates skin barrier protein expression.
ジャーナル Mol Med Rep
Abstract In the present study, the inhibitory effect of arazyme on allergic inflammation was investigated by evaluating the alteration of cytokine production and expression of skin barrier proteins in immune and HaCaT human keratinocyte cells. THP‑1 human monocytic and EoL‑1 human eosinophilic cells were treated with Dermatophagoides pteronissinus extract (DpE). Monocyte chemotactic protein‑1 (MCP‑1)/CCL2, interleukin (IL)‑6 and IL‑8 increased following DpE treatment and arazyme significantly blocked the increase of MCP‑1, IL‑6 and IL‑8 expression in cell types. Secretion of MCP‑1, IL‑6 and IL‑8 induced by lipopolysaccharide in THP‑1 cells was also inhibited by arazyme treatment. Arazyme inhibited the secretion of IL‑6 and IL‑8 due to phorbol 12‑myristate 13‑acetate and calcium ionophores in human mast cells. Arazyme blocked the secretion of thymus and activation‑regulated chemokine (TARC)/CCL17, MCP‑1, IL‑6 and IL‑8 due to tumor necrosis factor‑α (TNF‑α) and interferon‑γ (IFN‑γ) in HaCaT cells. TNF‑α and IFN‑γ suppressed the expression of skin barrier proteins, including filaggrin, involucrin and loricrin. By contrast, arazyme increased the expression of filaggrin, involucrin and loricrin. These results may contribute to the development of a therapeutic drug for the treatment of allergic diseases, including atopic dermatitis.
巻・号 8(2)
ページ 551-6
公開日 2013-8-1
DOI 10.3892/mmr.2013.1520
PMID 23764888
MeSH Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Line Chemokine CCL2 / biosynthesis Cytokines / genetics Cytokines / metabolism* Enterobacteriaceae / metabolism Eosinophils / drug effects Eosinophils / metabolism Eosinophils / pathology Filaggrin Proteins Gene Expression Regulation / drug effects Humans Interleukin-8 / biosynthesis Intermediate Filament Proteins / metabolism Keratinocytes / drug effects Keratinocytes / metabolism Membrane Proteins / metabolism Metalloproteases / isolation & purification Metalloproteases / pharmacology* Monocytes / drug effects Monocytes / immunology Monocytes / metabolism Protein Precursors / metabolism Skin / drug effects* Skin / immunology Skin / metabolism* Up-Regulation
IF 2.1
引用数 7
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL ONCOLOGY
リソース情報
ヒト・動物細胞