RRC ID 44481
Author Monzen S, Takimura K, Kashiwakura I, Hosokawa Y.
Title Acute promyelocytic leukemia mutated to radioresistance suppressed monocyte lineage differentiation by phorbol 12-myristate 13-acetate.
Journal Leuk. Res.
Abstract Induction of myeloid differentiation in radioresistant HL60 cells (Res-HL60) was examined to clarify the developmental mechanism of radioresistant leukemia. Compared to wild-type HL60 cells (Wt-HL60), Res-HL60 were smaller and strongly expressed CD38. Under all-trans retinoic acid (ATRA) stimulation, Res-HL60 continued to proliferate slowly and with similar level of CD11b expression to Wt-HL60. Phorbol 12-myristate 13-acetate (PMA) strongly suppressed proliferation of Res-HL60, downregulated CD14, and affected mRNA expression. These results suggested that the specific myeloid differentiation of Res-HL60 suppressed monocyte lineage by ATRA and PMA occurred through regulation of mRNA expression.
Volume 37(9)
Pages 1162-9
Published 2013-9
DOI 10.1016/j.leukres.2013.04.027
PII S0145-2126(13)00149-5
PMID 23773897
MeSH Antineoplastic Agents / pharmacology Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism Carcinogens / pharmacology Cell Differentiation / drug effects* Cell Differentiation / radiation effects* Cell Lineage* Flow Cytometry Gene Expression Profiling HL-60 Cells Humans Leukemia, Promyelocytic, Acute / drug therapy Leukemia, Promyelocytic, Acute / pathology* Leukemia, Promyelocytic, Acute / radiotherapy Monocytes / cytology Monocytes / drug effects Monocytes / radiation effects Mutation / genetics* Oligonucleotide Array Sequence Analysis Radiation Tolerance / drug effects* Tetradecanoylphorbol Acetate / pharmacology* Tretinoin / pharmacology Tumor Cells, Cultured X-Rays
IF 2.066
Times Cited 1
Human and Animal Cells