RRC ID |
44485
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著者 |
Ishizuka T, Goshima H, Ozawa A, Watanabe Y.
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タイトル |
Stimulation of α₁-adrenoceptor or angiotensin type 1 receptor enhances DNA synthesis in human-induced pluripotent stem cells via Gq-coupled receptor-dependent signaling pathways.
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ジャーナル |
Eur J Pharmacol
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Abstract |
Stimulation of either α₁-adrenoceptor or angiotensin type 1 receptor (AT₁ receptor) induces proliferation of mouse induced pluripotent stem (iPS) cells. Both α₁-adrenoceptor and AT₁ receptor are guanine nucleotide-binding protein q polypeptide (Gq)-coupled receptors. However, it is not fully understood whether stimulation of these Gq-coupled receptors exert a similar effect in human iPS cells, i.e. proliferation of human iPS cells. In this study, we evaluated the involvement of α₁-adrenoceptor and AT₁ receptor in the DNA synthesis of human iPS cells. Treatment with either l-phenylephrine (a selective α₁-adrenoceptor agonist) or angiotensin II (Ang II) significantly increased DNA synthesis in human iPS cells. Enhanced DNA synthesis was significantly inhibited by pretreatment with protein kinase C (PKC) inhibitors, mitogen-activated protein kinase kinase (MEK) inhibitor, or phosphatidylinositol-3 phosphate kinase (PI3K) inhibitor. Treatment with either l-phenylephrine or Ang II significantly increased Akt and p44/42 MAPK phosphorylation. Short interfering RNA (siRNA) directed against Gq significantly inhibited DNA synthesis and phosphorylation of Akt and p44/42 MAPK enhanced by l-phenylephrine or Ang II. These results suggest that stimulation of α₁-adrenoceptor or AT₁ receptor may enhance DNA synthesis in human iPS cells via Gq-coupled receptor-dependent signaling pathways.
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巻・号 |
714(1-3)
|
ページ |
202-9
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公開日 |
2013-8-15
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DOI |
10.1016/j.ejphar.2013.06.003
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PII |
S0014-2999(13)00469-X
|
PMID |
23792040
|
MeSH |
Adrenergic alpha-1 Receptor Agonists / pharmacology
Adrenergic alpha-1 Receptor Antagonists / pharmacology
Angiotensin II / pharmacology
Angiotensin II Type 1 Receptor Blockers / pharmacology
Animals
Cell Line
DNA / biosynthesis*
GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism*
Gene Expression Regulation / drug effects
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects
Induced Pluripotent Stem Cells / metabolism*
Mice
Phosphorylation / drug effects
Receptor, Angiotensin, Type 1 / metabolism*
Receptors, Adrenergic, alpha-1 / metabolism*
Signal Transduction / drug effects*
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IF |
3.263
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引用数 |
5
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WOS 分野
|
PHARMACOLOGY & PHARMACY
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リソース情報 |
ヒト・動物細胞 |
|