| RRC ID |
44490
|
| 著者 |
Okumura S, Ishikawa T, Saitoh H, Akao T, Mizuki E.
|
| タイトル |
Identification of a second cytotoxic protein produced by Bacillus thuringiensis A1470.
|
| ジャーナル |
Biotechnol Lett
|
| Abstract |
Bacillus thuringiensis A1470 produces multiple proteins with similar molecular masses (~30 kDa) with cytotoxicity against human cell lines. One that was previously identified, parasporin-4, is a β-pore-forming toxin. The N-terminal sequence of a second cytotoxic protein was identical to a partial sequence of parasporin-2 produced by B. thuringiensis A1547. PCR was performed on total plasmid DNA from A1470 by using primers for parasporin-2 to amplify a gene which was then cloned. The cloned gene differed from A1547 parasporin-2 by 8 bp and the predicted protein differed by four amino acids. The gene was expressed in Escherichia coli, and the cytotoxic activities of the recombinant protein against four human cell lines (MOLT-4, Jurkat, HeLa, and HepG2) were similar to those of A1547 parasporin-2. We then confirmed that the A1470 strain simultaneously produces parasporin-2 and parasporin-4.
|
| 巻・号 |
35(11)
|
| ページ |
1889-94
|
| 公開日 |
2013-11-1
|
| DOI |
10.1007/s10529-013-1275-6
|
| PMID |
23801126
|
| MeSH |
Bacillus thuringiensis / genetics
Bacillus thuringiensis / metabolism*
Bacterial Toxins / genetics
Bacterial Toxins / metabolism*
Cell Line
Cloning, Molecular
Cytotoxins / genetics
Cytotoxins / metabolism*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Escherichia coli / genetics
Gene Expression
Humans
Molecular Sequence Data
Plasmids
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / toxicity
Sequence Analysis, DNA
|
| IF |
1.977
|
| 引用数 |
7
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MOLT-4(RCB0206)
Jurkat
HeLa
Hep G2 |
