Reference - Detail
| RRC ID | 44492 |
|---|---|
| Author | Yamamoto M, Fujita H, Katase N, Inoue K, Nagatsuka H, Utsumi K, Sasaki J, Ohuchi H. |
| Title | Improvement of the efficacy of 5-aminolevulinic acid-mediated photodynamic treatment in human oral squamous cell carcinoma HSC-4. |
| Journal | Acta Med Okayama |
| Abstract |
Ever since protoporphyrin IX (PpIX) was discovered to accumulate preferentially in cancer cells after 5-aminolevulinic acid (ALA) treatment, photodynamic treatment or therapy (PDT) has been developed as an exciting new treatment option for cancer patients. However, the level of PpIX accumulation in oral cancer is fairly low and insufficient for PDT. Ferrochelatase (FECH) and ATP-binding cassette transporter G2 (ABCG2) are known to regulate PpIX accumulation. In addition, serum enhances PpIX export by ABCG2. We investigated here whether and how inhibitors of FECH and ABCG2 and their combination could improve PpIX accumulation and PDT efficacy in an oral cancer cell line in serum-containing medium. ABCG2 inhibitor and the combination of ABCG2 and FECH inhibitors increased PpIX in the presence of fetal bovine serum (FBS) in an oral cancer cell line. Analysis of ABCG2 gene silencing also revealed the involvement of ABCG2 in the regulation of PpIX accumulation. Inhibitors of FECH and ABCG2, and their combination increased the efficiency of ALA-PDT even in the presence of FBS. ALA-PDT-induced cell death was accompanied by apoptotic events and lipid peroxidation. These results suggest that accumulation of PpIX is determined by the activities of ABCG2 and FECH and that treatment with a combination of their inhibitors improves the efficacy of PDT for oral cancer, especially in the presence of serum. |
| Volume | 67(3) |
| Pages | 153-64 |
| Published | 2013-1-1 |
| DOI | 10.18926/AMO/50408 |
| PMID | 23804138 |
| MeSH | ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters / genetics Amino Acid Chloromethyl Ketones / pharmacology Aminolevulinic Acid / pharmacology* Apoptosis / drug effects Blood Proteins / pharmacology Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology Caspase Inhibitors / pharmacology Cell Line, Tumor Deferoxamine / pharmacology Ferrochelatase / antagonists & inhibitors Gene Silencing Humans Lipid Peroxidation / drug effects Mouth Neoplasms / drug therapy* Mouth Neoplasms / metabolism Mouth Neoplasms / pathology Neoplasm Proteins / genetics Photochemotherapy / methods* Photosensitizing Agents / pharmacology Protoporphyrins / metabolism Siderophores / pharmacology |
| IF | 0.642 |
| Times Cited | 13 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | |