Reference - Detail
| RRC ID | 44499 |
|---|---|
| Author | Miyashita S, Sagane Y, Niwa K, Watanabe T. |
| Title | Transport of the botulinum neurotoxin-associating protein, nontoxic nonhemagglutinin, across the rat small intestinal epithelial cell monolayer. |
| Journal | FEMS Microbiol Lett |
| Abstract |
Botulinum neurotoxin (BoNT) associates with nontoxic nonhemagglutinin (NTNHA) yielding a complex in culture. BoNT and NTNHA have similar domain organizations, implying that they share common functions, although this remains unclear. Here, we examined cell monolayer transport of serotype D NTNHA in the rat intestinal epithelial cell line IEC-6. NTNHA and BoNT both bound to the cell and were transported across the cell layer. NTNHA contains a QXW motif and a β-trefoil fold, both common in sugar chain-recognizing proteins, whereas the QXW motif is absent in all BoNT serotypes. This could explain the distinct sugar chain-recognizing properties of NTNHA and BoNT. |
| Volume | 346(1) |
| Pages | 73-80 |
| Published | 2013-9-1 |
| DOI | 10.1111/1574-6968.12205 |
| PMID | 23809018 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Animals Bacterial Proteins / metabolism* Botulinum Toxins / metabolism Cell Line Epithelial Cells / metabolism* Microscopy, Confocal Microscopy, Fluorescence Models, Molecular Molecular Sequence Data Protein Binding Protein Conformation Protein Transport Rats Sequence Alignment |
| IF | 1.987 |
| Times Cited | 4 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | IEC 6(RCB0993) |