論文 - 詳細
| RRC ID | 4453 |
|---|---|
| 著者 | Kim KW, Nykamp K, Suh N, Bachorik JL, Wang L, Kimble J. |
| タイトル | Antagonism between GLD-2 binding partners controls gamete sex. |
| ジャーナル | Dev Cell |
| Abstract |
Cytoplasmic polyadenylation is a key mechanism of gene control. In Caenorhabditis elegans, GLD-2 and GLD-3 provide the catalytic and RNA-binding subunits, respectively, of a major cytoplasmic poly(A) polymerase (PAP). Here, we identify RNP-8 as a second GLD-2 partner. RNP-8 binds GLD-2 and stimulates GLD-2 activity to form a functional PAP, much like GLD-3. Moreover, GLD-2/RNP-8 and GLD-2/GLD-3 exist as separate complexes that form selectively during development, and RNP-8 and GLD-3 appear to have distinct RNA-binding specificities. Therefore, GLD-2 can form either of two discrete PAPs. In C. elegans hermaphrodites, gamete production begins with spermatogenesis and transitions later to oogenesis. We suggest that the combinatorial use of GLD-2 contributes to this transition, as GLD-2/GLD-3 promotes spermatogenesis, whereas GLD-2/RNP-8 specifies oogenesis. Indeed, RNP-8 and GLD-3 antagonize each other, as evidenced by genetic cosuppression and molecular competition for GLD-2 binding. We conclude that GLD-2 and its binding partners control gamete identity. |
| 巻・号 | 16(5) |
| ページ | 723-33 |
| 公開日 | 2009-5-1 |
| DOI | 10.1016/j.devcel.2009.04.002 |
| PII | S1534-5807(09)00138-5 |
| PMID | 19460348 |
| PMC | PMC2728548 |
| MeSH | Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / metabolism* Disorders of Sex Development Gametogenesis Polynucleotide Adenylyltransferase / metabolism* RNA-Binding Proteins / metabolism* |
| IF | 10.092 |
| 引用数 | 41 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2435 |