RRC ID 4453
Author Kim KW, Nykamp K, Suh N, Bachorik JL, Wang L, Kimble J.
Title Antagonism between GLD-2 binding partners controls gamete sex.
Journal Dev. Cell
Abstract Cytoplasmic polyadenylation is a key mechanism of gene control. In Caenorhabditis elegans, GLD-2 and GLD-3 provide the catalytic and RNA-binding subunits, respectively, of a major cytoplasmic poly(A) polymerase (PAP). Here, we identify RNP-8 as a second GLD-2 partner. RNP-8 binds GLD-2 and stimulates GLD-2 activity to form a functional PAP, much like GLD-3. Moreover, GLD-2/RNP-8 and GLD-2/GLD-3 exist as separate complexes that form selectively during development, and RNP-8 and GLD-3 appear to have distinct RNA-binding specificities. Therefore, GLD-2 can form either of two discrete PAPs. In C. elegans hermaphrodites, gamete production begins with spermatogenesis and transitions later to oogenesis. We suggest that the combinatorial use of GLD-2 contributes to this transition, as GLD-2/GLD-3 promotes spermatogenesis, whereas GLD-2/RNP-8 specifies oogenesis. Indeed, RNP-8 and GLD-3 antagonize each other, as evidenced by genetic cosuppression and molecular competition for GLD-2 binding. We conclude that GLD-2 and its binding partners control gamete identity.
Volume 16(5)
Pages 723-33
Published 2009-5
DOI 10.1016/j.devcel.2009.04.002
PII S1534-5807(09)00138-5
PMID 19460348
PMC PMC2728548
MeSH Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / metabolism* Disorders of Sex Development Gametogenesis Polynucleotide Adenylyltransferase / metabolism* RNA-Binding Proteins / metabolism*
IF 9.19
Times Cited 30
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
C.elegans tm2435