| RRC ID |
44549
|
| 著者 |
Meshii N, Takahashi G, Okunaga S, Hamada M, Iwai S, Takasu A, Ogawa Y, Yura Y.
|
| タイトル |
Enhancement of systemic tumor immunity for squamous cell carcinoma cells by an oncolytic herpes simplex virus.
|
| ジャーナル |
Cancer Gene Ther
|
| Abstract |
RH2 is a neurovirulent γ134.5 gene-deficient herpes simplex virus type 1 (HSV-1) with a lytic ability in human squamous cell carcinoma (SCC) cells; it is related to spontaneously occurring HSV-1 mutant HF10. The effect of RH2 on SCC was examined using a syngeneic C3H mouse model. After infection of mouse SCCVII cells with RH2, cell viability was decreased at first, but recovered by prolonged culture, indicating the limited replication of RH2. The antitumor ability of RH2 was examined using a bilateral SCCVII tumor model. The growth of the RH2-injected tumors was suppressed compared with that of phosphate-buffered saline-injected tumors. Moreover, the growth of contralateral tumor of RH2-treated mice was also suppressed significantly. The splenocytes of C3H mice treated with RH2 lysed more SCCVII cells than NFSaY83 cells and YAC-1 cells. The cytotoxicity of the splenocytes on SCCVII cells was significantly greater than that of splenocytes from tumor-bearing mice. Removal of CD8(+) T cells from splenocytes decreased their cell killing activity remarkably. The antitumor effect of RH2 on SCCVII xenografts in nude mice was not demonstrated. These results indicate that RH2 exhibited a suppressive effect on mouse SCC, even if the replication of RH2 was limited. This is ascribed to the ability of RH2 to enhance existing tumor-specific cytotoxic T lymphocyte activity.
|
| 巻・号 |
20(9)
|
| ページ |
493-8
|
| 公開日 |
2013-9-1
|
| DOI |
10.1038/cgt.2013.45
|
| PII |
cgt201345
|
| PMID |
23887644
|
| MeSH |
Animals
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / immunology*
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Cytotoxicity, Immunologic
Disease Models, Animal
Female
Gene Expression
Genetic Therapy
Genetic Vectors / genetics
Genetic Vectors / immunology
Herpesvirus 1, Human / genetics
Herpesvirus 1, Human / immunology*
Humans
Lymphocyte Depletion
Lymphocyte Subsets / immunology
Mice
Mice, Nude
Neoplasms / genetics
Neoplasms / immunology*
Neoplasms / pathology
Oncolytic Viruses / genetics
Oncolytic Viruses / immunology*
Spleen / cytology
Spleen / immunology
Transduction, Genetic
Transgenes
Tumor Burden / genetics
|
| IF |
4.534
|
| 引用数 |
18
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
NFSa Y83(RCB0282)
YAC-1(RCB1165) |
