論文 - 詳細
| RRC ID | 44573 |
|---|---|
| 著者 | Shimomura S, Matsuno H, Tanaka K. |
| タイトル | Effect of mechanical instability of polymer scaffolds on cell adhesion. |
| ジャーナル | Langmuir |
| Abstract |
The adhesion of fibroblast on polymer bilayers composed of a glassy polystyrene (PS) prepared on top of a rubbery polyisoprene (PI) was studied. Since the top PS layer is not build on a glassy, or firm, foundation, the system becomes mechanically unstable with decreasing thickness of the PS layer. When the PS film was thinner than 25 nm, the number of cells adhered to the surface decreased and the cells could not spread well. On a parallel experiment, the same cell adhesion behavior was observed on plasma-treated PS/PI bilayer films, where in this case, the surface was more hydrophilic than that of the intact films. In addition, the fluorescence microscopic observations revealed that the formation of F-actin filaments in fibroblasts attached to the thicker PS/PI bilayer films was greater than those using the thinner PS/PI bilayer films. On the other hand, the thickness dependence of the cell adhesion behavior was not observed for the PS monolayer films. Taking into account that the amount of adsorbed protein molecules evaluated by a quartz crystal microbalance method was independent of the PS layer thickness of the bilayer films, our results indicate that cells, unlike protein molecules, could sense a mechanical instability of the scaffold. |
| 巻・号 | 29(35) |
| ページ | 11087-92 |
| 公開日 | 2013-9-3 |
| DOI | 10.1021/la4027706 |
| PMID | 23919741 |
| MeSH | Actin Cytoskeleton / ultrastructure Actins / chemistry Adsorption Animals Butadienes / chemistry Cell Adhesion Cell Line Fibroblasts / drug effects* Fibroblasts / physiology Fibroblasts / ultrastructure Humans Hydrophobic and Hydrophilic Interactions Immunoglobulin G / chemistry Mice Microscopy, Fluorescence Polystyrenes / chemistry Polystyrenes / pharmacology* Quartz Crystal Microbalance Techniques Serum Albumin / chemistry Surface Properties Tissue Scaffolds / chemistry* |
| IF | 3.557 |
| 引用数 | 15 |
| WOS 分野 | MATERIALS SCIENCE, MULTIDISCIPLINARY CHEMISTRY, MULTIDISCIPLINARY CHEMISTRY, PHYSICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929 |