RRC ID 44578
Author Nishimura Y, Lee H, Hafenstein S, Kataoka C, Wakita T, Bergelson JM, Shimizu H.
Title Enterovirus 71 binding to PSGL-1 on leukocytes: VP1-145 acts as a molecular switch to control receptor interaction.
Journal PLoS Pathog
Abstract Some strains of enterovirus 71 (EV71), but not others, infect leukocytes by binding to a specific receptor molecule: the P-selectin glycoprotein ligand-1 (PSGL-1). We find that a single amino acid residue within the capsid protein VP1 determines whether EV71 binds to PSGL-1. Examination of capsid sequences of representative EV71 strains revealed that the PSGL-1-binding viruses had either a G or a Q at residue 145 within the capsid protein VP1 (VP1-145G or Q), whereas PSGL-1-nonbinding viruses had VP1-145E. Using site-directed mutagenesis we found that PSGL-1-binding strains lost their capacity to bind when VP1-145G/Q was replaced by E; conversely, nonbinding strains gained the capacity to bind PSGL-1 when VP1-145E was replaced with either G or Q. Viruses with G/Q at VP1-145 productively infected a leukocyte cell line, Jurkat T-cells, whereas viruses with E at this position did not. We previously reported that EV71 binds to the N-terminal region of PSGL-1, and that binding depends on sulfated tyrosine residues within this region. We speculated that binding depends on interaction between negatively charged sulfate groups and positively charged basic residues in the virus capsid. VP1-145 on the virus surface is in close proximity to conserved lysine residues at VP1-242 and VP1-244. Comparison of recently published crystal structures of EV71 isolates with either Q or E at VP1-145 revealed that VP1-145 controls the orientation of the lysine side-chain of VP1-244: with VP1-145Q the lysine side chain faces outward, but with VP1-145E, the lysine side chain is turned toward the virus surface. Mutation of VP1-244 abolished virus binding to PSGL-1, and mutation of VP1-242 greatly reduced binding. We propose that conserved lysine residues on the virus surface are responsible for interaction with sulfated tyrosine residues at the PSGL-1 N-terminus, and that VP1-145 acts as a switch, controlling PSGL-1 binding by modulating the exposure of VP1-244K.
Volume 9(7)
Pages e1003511
Published 2013
DOI 10.1371/journal.ppat.1003511
PII PPATHOGENS-D-13-00757
PMID 23935488
PMC PMC3723564
MeSH Amino Acid Sequence Amino Acid Substitution Binding Sites Capsid Proteins / chemistry Capsid Proteins / genetics Capsid Proteins / metabolism* Conserved Sequence Enterovirus A, Human / immunology* Enterovirus A, Human / physiology Host-Pathogen Interactions Humans Immunoglobulin Fc Fragments / chemistry Immunoglobulin Fc Fragments / genetics Immunoglobulin Fc Fragments / metabolism Jurkat Cells Leukocytes / immunology* Leukocytes / metabolism Leukocytes / virology Lysine / chemistry Membrane Glycoproteins / chemistry Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Mutagenesis, Site-Directed Mutant Proteins / chemistry Mutant Proteins / metabolism Protein Interaction Domains and Motifs Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / metabolism Tyrosine / analogs & derivatives Tyrosine / chemistry Viral Fusion Proteins / chemistry Viral Fusion Proteins / genetics Viral Fusion Proteins / metabolism* Virus Attachment
IF 6.463
Times Cited 67
WOS Category PARASITOLOGY MICROBIOLOGY VIROLOGY
Resource
Human and Animal Cells