| RRC ID |
44582
|
| 著者 |
Zhang J, Lazarenko OP, Kang J, Blackburn ML, Ronis MJ, Badger TM, Chen JR.
|
| タイトル |
Feeding blueberry diets to young rats dose-dependently inhibits bone resorption through suppression of RANKL in stromal cells.
|
| ジャーナル |
PLoS One
|
| Abstract |
Previous studies have demonstrated that weanling rats fed AIN-93G semi-purified diets supplemented with 10% whole blueberry (BB) powder for two weeks beginning on postnatal day 21 (PND21) significantly increased bone formation at PND35. However, the minimal level of dietary BB needed to produce these effects is, as yet, unknown. The current study examined the effects of three different levels of BB diet supplementation (1, 3, and 5%) for 35 days beginning on PND25 on bone quality, and osteoclastic bone resorption in female rats. Peripheral quantitative CT scan (pQCT) of tibia, demonstrated that bone mineral density (BMD) and content (BMC) were dose-dependently increased in BB-fed rats compared to controls (P<0.05). Significantly increased bone mass after feeding 5% BB extracts was also observed in a TEN (total enteral nutrition) rat model in which daily caloric and food intake was precisely controlled. Expression of RANKL (receptor activator of nuclear factor-κB ligand) a protein essential for osteoclast formation was dose-dependently decreased in the femur of BB animals. In addition, expression of PPARγ (peroxisome proliferator-activated receptor γ) which regulates bone marrow adipogenesis was suppressed in BB diet rats compared to non-BB diet controls. Finally, a set of in vitro cell cultures revealed that the inhibitory effect of BB diet rat serum on RANKL expression was more profound in mesenchymal stromal cells compared to its effect on mature osteoblasts, pre-adipocytes and osteocytes. These results suggest that inhibition of bone resorption may contribute to increased bone mass during early development after BB consumption.
|
| 巻・号 |
8(8)
|
| ページ |
e70438
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0070438
|
| PII |
PONE-D-13-10815
|
| PMID |
23936431
|
| PMC |
PMC3735613
|
| MeSH |
Adipocytes / pathology
Animal Feed
Animals
Blueberry Plants*
Bone Marrow Cells / pathology
Bone Resorption / diet therapy*
Bone Resorption / metabolism*
Bone Resorption / pathology
Bone and Bones / metabolism
Bone and Bones / pathology
Cell Differentiation
Cell Line
Diet*
Dietary Supplements*
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Gene Expression Regulation
Organ Size
Osteoclasts / pathology
Osteoprotegerin / metabolism
PPAR gamma / genetics
RANK Ligand / metabolism*
Rats
Rats, Sprague-Dawley
Stromal Cells / metabolism*
|
| IF |
2.74
|
| 引用数 |
19
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
