RRC ID 44582
Author Zhang J, Lazarenko OP, Kang J, Blackburn ML, Ronis MJ, Badger TM, Chen JR.
Title Feeding blueberry diets to young rats dose-dependently inhibits bone resorption through suppression of RANKL in stromal cells.
Journal PLoS One
Abstract Previous studies have demonstrated that weanling rats fed AIN-93G semi-purified diets supplemented with 10% whole blueberry (BB) powder for two weeks beginning on postnatal day 21 (PND21) significantly increased bone formation at PND35. However, the minimal level of dietary BB needed to produce these effects is, as yet, unknown. The current study examined the effects of three different levels of BB diet supplementation (1, 3, and 5%) for 35 days beginning on PND25 on bone quality, and osteoclastic bone resorption in female rats. Peripheral quantitative CT scan (pQCT) of tibia, demonstrated that bone mineral density (BMD) and content (BMC) were dose-dependently increased in BB-fed rats compared to controls (P<0.05). Significantly increased bone mass after feeding 5% BB extracts was also observed in a TEN (total enteral nutrition) rat model in which daily caloric and food intake was precisely controlled. Expression of RANKL (receptor activator of nuclear factor-κB ligand) a protein essential for osteoclast formation was dose-dependently decreased in the femur of BB animals. In addition, expression of PPARγ (peroxisome proliferator-activated receptor γ) which regulates bone marrow adipogenesis was suppressed in BB diet rats compared to non-BB diet controls. Finally, a set of in vitro cell cultures revealed that the inhibitory effect of BB diet rat serum on RANKL expression was more profound in mesenchymal stromal cells compared to its effect on mature osteoblasts, pre-adipocytes and osteocytes. These results suggest that inhibition of bone resorption may contribute to increased bone mass during early development after BB consumption.
Volume 8(8)
Pages e70438
Published 2013-1-1
DOI 10.1371/journal.pone.0070438
PII PONE-D-13-10815
PMID 23936431
PMC PMC3735613
MeSH Adipocytes / pathology Animal Feed Animals Blueberry Plants* Bone Marrow Cells / pathology Bone Resorption / diet therapy* Bone Resorption / metabolism* Bone Resorption / pathology Bone and Bones / metabolism Bone and Bones / pathology Cell Differentiation Cell Line Diet* Dietary Supplements* Disease Models, Animal Dose-Response Relationship, Drug Female Gene Expression Regulation Organ Size Osteoclasts / pathology Osteoprotegerin / metabolism PPAR gamma / genetics RANK Ligand / metabolism* Rats Rats, Sprague-Dawley Stromal Cells / metabolism*
IF 2.74
Times Cited 19
Human and Animal Cells ST2(RCB0224)