論文 - 詳細
| RRC ID | 44615 |
|---|---|
| 著者 | Wu Z, Zhu A, Takayama F, Okada R, Liu Y, Harada Y, Wu S, Nakanishi H. |
| タイトル | Brazilian green propolis suppresses the hypoxia-induced neuroinflammatory responses by inhibiting NF-κB activation in microglia. |
| ジャーナル | Oxid Med Cell Longev |
| Abstract |
Hypoxia has been recently proposed as a neuroinflammatogen, which drives microglia to produce proinflammatory cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and IL-6. Considering the fact that propolis has hepatoprotective, antitumor, antioxidative, and anti-inflammatory effects, propolis may have protective effects against the hypoxia-induced neuroinflammatory responses. In this study, propolis (50 μg/mL) was found to significantly inhibit the hypoxia-induced cytotoxicity and the release of proinflammatory cytokines, including IL-1β, TNF-α, and IL-6, by MG6 microglia following hypoxic exposure (1% O2, 24 h). Furthermore, propolis significantly inhibited the hypoxia-induced generation of reactive oxygen species (ROS) from mitochondria and the activation of nuclear factor-κB (NF-κB) in microglia. Moreover, systemic treatment with propolis (8.33 mg/kg, 2 times/day, i.p.) for 7 days significantly suppressed the microglial expression of IL-1β, TNF-α, IL-6, and 8-oxo-deoxyguanosine, a biomarker for oxidative damaged DNA, in the somatosensory cortex of mice subjected to hypoxia exposure (10% O2, 4 h). These observations indicate that propolis suppresses the hypoxia-induced neuroinflammatory responses through inhibition of the NF-κB activation in microglia. Furthermore, increased generation of ROS from the mitochondria is responsible for the NF-κB activation. Therefore, propolis may be beneficial in preventing hypoxia-induced neuroinflammation. |
| 巻・号 | 2013 |
| ページ | 906726 |
| 公開日 | 2013-1-1 |
| DOI | 10.1155/2013/906726 |
| PMID | 23983903 |
| PMC | PMC3747398 |
| MeSH | Animals Cell Hypoxia / drug effects Cell Hypoxia / physiology* Cell Line Cell Survival / drug effects Electrophoresis Enzyme-Linked Immunosorbent Assay Immunoblotting Mice Microglia / cytology Microglia / drug effects* Microglia / metabolism* NF-kappa B / metabolism Propolis / antagonists & inhibitors* Propolis / pharmacology* Reactive Oxygen Species / metabolism |
| IF | 5.076 |
| 引用数 | 12 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MG6(RCB2403) |