論文 - 詳細
| RRC ID | 44619 |
|---|---|
| 著者 | Li Y, Miyanari Y, Shirane K, Nitta H, Kubota T, Ohashi H, Okamoto A, Sasaki H. |
| タイトル | Sequence-specific microscopic visualization of DNA methylation status at satellite repeats in individual cell nuclei and chromosomes. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Methylation-specific fluorescence in situ hybridization (MeFISH) was developed for microscopic visualization of DNA methylation status at specific repeat sequences in individual cells. MeFISH is based on the differential reactivity of 5-methylcytosine and cytosine in target DNA for interstrand complex formation with osmium and bipyridine-containing nucleic acids (ICON). Cell nuclei and chromosomes hybridized with fluorescence-labeled ICON probes for mouse major and minor satellite repeats were treated with osmium for crosslinking. After denaturation, fluorescent signals were retained specifically at satellite repeats in wild-type, but not in DNA methyltransferase triple-knockout (negative control) mouse embryonic stem cells. Moreover, using MeFISH, we successfully detected hypomethylated satellite repeats in cells from patients with immunodeficiency, centromeric instability and facial anomalies syndrome and 5-hydroxymethylated satellite repeats in male germ cells, the latter of which had been considered to be unmethylated based on anti-5-methylcytosine antibody staining. MeFISH will be suitable for a wide range of applications in epigenetics research and medical diagnosis. |
| 巻・号 | 41(19) |
| ページ | e186 |
| 公開日 | 2013-10-1 |
| DOI | 10.1093/nar/gkt766 |
| PII | gkt766 |
| PMID | 23990328 |
| PMC | PMC3799461 |
| MeSH | 5-Methylcytosine / analogs & derivatives Animals Base Sequence Cell Nucleus / genetics* Cells, Cultured Chromosomes, Mammalian / chemistry* Cytosine / analogs & derivatives Cytosine / analysis DNA Methylation* DNA, Satellite* Embryonic Stem Cells / chemistry Humans In Situ Hybridization, Fluorescence / methods* Male Mice Spermatozoa / chemistry |
| IF | 11.502 |
| 引用数 | 19 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | HEV0190 |