RRC ID 44625
著者 Li Q, Jie Y, Wang C, Zhang Y, Guo H, Pan Z.
タイトル Tryptase compromises corneal epithelial barrier function.
ジャーナル Cell Biochem Funct
Abstract Corneal epithelial barrier dysfunction is harmful to corneal health; the pathogenesis is unclear. This study aims to elucidate the mechanism by which tryptase compromises corneal epithelial barrier function. Human corneal epithelial cell line (HCE cells) was cultured into monolayers using as a study platform. Quantitative reverse transcription polymerase chain reaction and Western blotting were employed to detect the expression of matrix metalloprotenases (MMP)9. The endosome/lysosome fusion was observed by confocal microscopy. The corneal epithelial barrier function was assessed in Transwell system. The results showed that HCE cells expressed proteinase-activated receptor (PAR)2. Activation of PAR2 by tryptase induced expression of MMP9 in HCE cells, interfered with the fusion of endosome/lysosome, and compromised the epithelial barrier function, which could be prevented by pretreatment with MMP9 inhibitor. We conclude that tryptase can increase the expression of MMP9 in HCE cells and compromise the epithelial barrier function.
巻・号 32(2)
ページ 183-7
公開日 2014-3-1
DOI 10.1002/cbf.2991
PMID 23996353
MeSH Cell Line Endosomes / physiology Epithelium, Corneal / cytology Epithelium, Corneal / metabolism* Gene Silencing Humans Lysosomes / physiology Matrix Metalloproteinase 9 / genetics Matrix Metalloproteinase 9 / metabolism Matrix Metalloproteinase Inhibitors / pharmacology Membrane Fusion Receptor, PAR-2 / genetics Receptor, PAR-2 / metabolism Transcription, Genetic Tryptases / metabolism*
IF 2.632
引用数 8
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)