論文 - 詳細
| RRC ID | 44657 |
|---|---|
| 著者 | Matsui K, Oda K, Mizuta S, Ishino R, Urahama N, Hasegawa N, Roeder RG, Ito M. |
| タイトル | Mediator subunit MED1 is a T3-dependent and T3-independent coactivator on the thyrotropin β gene promoter. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The MED1 subunit of the Mediator transcriptional coregulator complex is a nuclear receptor-specific coactivator. A negative feedback mechanism of thyroid-stimulating hormone (TSH, or thyrotropin) expression in the thyrotroph in the presence of triiodothyronine (T3) is employed by liganded thyroid hormone receptor β (TRβ) on the TSHβ gene promoter, where conventional histone-modifying coactivators act as corepressors. We now provide evidence that MED1 is a ligand-dependent positive cofactor on this promoter. TSHβ gene transcription was attenuated in MED1 mutant mice in which the nuclear receptor-binding ability of MED1 was specifically disrupted. MED1 stimulated GATA2- and Pit1-mediated TSHβ gene promoter activity in a ligand-independent manner in cultured cells. MED1 also stimulated transcription from the TSHβ gene promoter in a T3-dependent manner. The transcription was further enhanced when the T3-dependent corepressors SRC1, SRC2, and HDAC2 were downregulated. Hence, MED1 is a T3-dependent and -independent coactivator on the TSHβ gene promoter. |
| 巻・号 | 440(1) |
| ページ | 184-9 |
| 公開日 | 2013-10-11 |
| DOI | 10.1016/j.bbrc.2013.09.061 |
| PII | S0006-291X(13)01542-8 |
| PMID | 24055033 |
| PMC | PMC4388257 |
| MeSH | Animals Cell Line Female GATA2 Transcription Factor / metabolism Humans Male Mediator Complex Subunit 1 / genetics Mediator Complex Subunit 1 / metabolism* Mice Mice, Inbred C57BL Mutation Promoter Regions, Genetic* Protein Subunits / genetics Protein Subunits / metabolism Thyrotropin, beta Subunit / genetics* Transcription Factor Pit-1 / metabolism Transcriptional Activation* Triiodothyronine / metabolism* |
| IF | 2.985 |
| 引用数 | 1 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | CV-1(RCB0160) |