RRC ID 44676
Author Tokunaga M, Shiheido H, Tabata N, Sakuma-Yonemura Y, Takashima H, Horisawa K, Doi N, Yanagawa H.
Title MIP-2A is a novel target of an anilinoquinazoline derivative for inhibition of tumour cell proliferation.
Journal PLoS One
Abstract We recently identified a novel anilinoquinazoline derivative, Q15, as a potent apoptosis inducer in a panel of human cancer cell lines and determined that Q15 targets hCAP-G2, a subunit of condensin II complex, leading to abnormal cell division. However, whether the defect in normal cell division directly results in cell death remains unclear. Here, we used an mRNA display method on a microfluidic chip to search for other Q15-binding proteins. We identified an additional Q15-binding protein, MIP-2A (MBP-1 interacting protein-2A), which has been reported to interact with MBP-1, a repressor of the c-Myc promoter. Our results indicate that Q15 inhibits the interaction between MIP-2A and MBP-1 as well as the expression of c-Myc protein, thereby inducing cell death. This study suggests that the simultaneous targeting of hCAP-G2 and MIP-2A is a promising strategy for the development of antitumor drugs as a treatment for intractable tumours.
Volume 8(9)
Pages e76774
Published 2013-1-1
DOI 10.1371/journal.pone.0076774
PII PONE-D-13-28614
PMID 24098805
PMC PMC3786957
MeSH Adenosine Triphosphatases / metabolism Amino Acid Sequence Aniline Compounds / chemistry Aniline Compounds / metabolism Aniline Compounds / pharmacology* Antineoplastic Agents / chemistry Antineoplastic Agents / metabolism Antineoplastic Agents / pharmacology* Apoptosis / drug effects* Cell Line, Tumor Cell Proliferation / drug effects* DNA-Binding Proteins / metabolism Gene Expression Profiling Humans Membrane Transport Proteins / metabolism* Microfluidic Analytical Techniques Molecular Sequence Data Molecular Structure Multiprotein Complexes / metabolism Quinazolines / chemistry Quinazolines / metabolism Quinazolines / pharmacology* Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / metabolism*
IF 2.74
Times Cited 4
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells 293T(RCB2202) HeLa