| RRC ID |
44694
|
| Author |
Nishimori M, Yakushiji H, Mori M, Miyamoto T, Yaguchi T, Ohno S, Miyake Y, Sakaguchi T, Ueda M, Ohno E.
|
| Title |
Tumorigenesis in cells derived from induced pluripotent stem cells.
|
| Journal |
Hum Cell
|
| Abstract |
Induced pluripotent stem (iPS) cells are an attractive source for potential cell-replacement therapy. However, transplantation of differentiated products harbors the risk of teratoma formation, presenting a serious health risk. Thus, we characterized Nanog-expressing (undifferentiated) cells remaining after induction of differentiation by cytological examination. To induce differentiation of iPS cells, we generated embryoid bodies (EBs) derived from iPS cells carrying a Nanog–green fluorescent protein(GFP) reporter and then injected GFP-positive and GFP negative EBs into nude mice. GFP-positive EB transplantation resulted in the formation of immature teratoma grade 3, but no tumors were induced by GFP-negative EB. GFP positive cells revealed significantly lower cytoplasmic area and higher nucleus/cytoplasm ratio than those of GFP negative cells. Our results suggest that morphological analysis might be a useful method for distinguishing between tumorigenic and nontumorigenic iPS cells.
|
| Volume |
27(1)
|
| Pages |
29-35
|
| Published |
2014-1-1
|
| DOI |
10.1007/s13577-013-0078-3
|
| PMID |
24122447
|
| MeSH |
Animals
Cell Differentiation
Cell Transformation, Neoplastic*
Cells, Cultured
Embryoid Bodies / cytology
Green Fluorescent Proteins
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / pathology*
Mice
Mice, Nude
Stem Cell Transplantation / adverse effects*
Teratoma / etiology*
Teratoma / pathology*
|
| IF |
3.463
|
| Times Cited |
22
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
iPS-MEF-Ng-20D-17(APS0001) |
