Reference - Detail
| RRC ID | 44712 |
|---|---|
| Author | Ito T, Kajino K, Abe M, Sato K, Maekawa H, Sakurada M, Orita H, Wada R, Kajiyama Y, Hino O. |
| Title | ERC/mesothelin is expressed in human gastric cancer tissues and cell lines. |
| Journal | Oncol Rep |
| Abstract |
ERC/mesothelin is expressed in mesothelioma and other malignancies. The ERC/mesothelin gene (MSLN) encodes a 71-kDa precursor protein, which is cleaved to yield 31-kDa N-terminal (N-ERC/mesothelin) and 40-kDa C-terminal (C-ERC/mesothelin) proteins. N-ERC/mesothelin is a soluble protein and has been reported to be a diagnostic serum marker of mesothelioma and ovarian cancer. Gastric cancer tissue also expresses C-ERC/mesothelin, but the significance of serum N-ERC levels for diagnosing gastric cancer has not yet been studied. We examined the latter issue in the present study as well as C-ERC/mesothelin expression in human gastric cancer tissues and cell lines. We immunohistochemically examined C-ERC/mesothelin expression in tissue samples from 50 cases of gastric cancer, and we also assessed the C-ERC/mesothelin expression in 6 gastric cancer cell lines (MKN-1, MKN-7, MKN-74, NUGC-3, NUGC-4 and TMK-1) using reverse transcription-polymerase chain reaction, flow cytometry, immunohistochemistry and immunoblotting. We also examined the N-ERC/mesothelin concentrations in the supernatants of cultured cells and in the sera of gastric cancer patients using an enzyme-linked immunosorbent assay (ELISA). N-ERC/mesothelin was detected in the supernatants of 3 gastric cancer cell lines (MKN-1, NUGC-4 and TMK-1) by ELISA, but its concentration in the sera of gastric cancer patients was almost same as that observed in the sera of the normal controls. In the gastric cancer tissues, C-ERC/mesothelin expression was associated with lymphatic invasion. N-ERC/mesothelin was secreted into the supernatants of gastric cancer cell lines, but does not appear to be a useful serum marker of gastric cancer. |
| Volume | 31(1) |
| Pages | 27-33 |
| Published | 2014-1-1 |
| DOI | 10.3892/or.2013.2803 |
| PMID | 24146039 |
| PMC | PMC3868502 |
| MeSH | Adult Aged Aged, 80 and over Biomarkers, Tumor / biosynthesis* Biomarkers, Tumor / blood Biomarkers, Tumor / genetics Cell Line, Tumor Female GPI-Linked Proteins / biosynthesis* GPI-Linked Proteins / blood GPI-Linked Proteins / genetics Gene Expression Humans Lymphatic Metastasis Male Mesothelin Middle Aged Stomach Neoplasms / blood Stomach Neoplasms / genetics Stomach Neoplasms / metabolism* |
| IF | 3.417 |
| Times Cited | 10 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MKN1(RCB1003) MKN7(RCB0999) MKN74(RCB1002) NUGC-4(RCB1939) |