論文 - 詳細
| RRC ID | 44716 |
|---|---|
| 著者 | Futamura Y, Kawatani M, Muroi M, Aono H, Nogawa T, Osada H. |
| タイトル | Identification of a molecular target of a novel fungal metabolite, pyrrolizilactone, by phenotypic profiling systems. |
| ジャーナル | Chembiochem |
| Abstract |
In the course of screening our microbial metabolite fraction library, we identified a novel pyrrolizidinone compound, pyrrolizilactone. In this study, we report the identification and characterization of a molecular target for pyrrolizilactone by using two phenotypic profiling systems. Cell morphology-based profiling analysis using an imaging cytometer (MorphoBase) classified pyrrolizilactone as a proteasome inhibitor. Consistently, proteome-based profiling analysis using 2D difference gel electrophoresis (DIGE; ChemProteoBase) also demonstrated that pyrrolizilactone is associated with proteasome inhibition. On the basis of these predictions, we determined that pyrrolizilactone is a novel type of proteasome inhibitor inhibiting the trypsin-like activity of the proteasome. |
| 巻・号 | 14(18) |
| ページ | 2456-63 |
| 公開日 | 2013-12-16 |
| DOI | 10.1002/cbic.201300499 |
| PMID | 24166831 |
| MeSH | Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Biological Products / chemistry Biological Products / pharmacology* Databases, Factual Fungi / chemistry* HeLa Cells Humans Lactones / chemistry Lactones / pharmacology* Molecular Targeted Therapy* Neoplasms / drug therapy Neoplasms / enzymology Proteasome Inhibitors / chemistry Proteasome Inhibitors / pharmacology* |
| IF | 2.576 |
| 引用数 | 18 |
| WOS 分野 | CHEMISTRY, MEDICINAL BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |