論文 - 詳細
| RRC ID | 44726 |
|---|---|
| 著者 | Kubo S, Takagi-Kimura M, Logg CR, Kasahara N. |
| タイトル | Highly efficient tumor transduction and antitumor efficacy in experimental human malignant mesothelioma using replicating gibbon ape leukemia virus. |
| ジャーナル | Cancer Gene Ther |
| Abstract |
Retroviral replicating vectors (RRVs) have been shown to achieve efficient tumor transduction and enhanced therapeutic benefit in a wide variety of cancer models. Here we evaluated two different RRVs derived from amphotropic murine leukemia virus (AMLV) and gibbon ape leukemia virus (GALV), in human malignant mesothelioma cells. In vitro, both RRVs expressing the green fluorescent protein gene efficiently replicated in most mesothelioma cell lines tested, but not in normal mesothelial cells. Notably, in ACC-MESO-1 mesothelioma cells that were not permissive for AMLV-RRV, the GALV-RRV could spread efficiently in culture and in mice with subcutaneous xenografts by in vivo fluorescence imaging. Next, GALV-RRV expressing the cytosine deaminase prodrug activator gene showed efficient killing of ACC-MESO-1 cells in a prodrug 5-fluorocytosine dose-dependent manner, compared with AMLV-RRV. GALV-RRV-mediated prodrug activator gene therapy achieved significant inhibition of subcutaneous ACC-MESO-1 tumor growth in nude mice. Quantitative reverse transcription PCR demonstrated that ACC-MESO-1 cells express higher PiT-1 (GALV receptor) and lower PiT-2 (AMLV receptor) compared with normal mesothelial cells and other mesothelioma cells, presumably accounting for the distinctive finding that GALV-RRV replicates much more robustly than AMLV-RRV in these cells. These data indicate the potential utility of GALV-RRV-mediated prodrug activator gene therapy in the treatment of mesothelioma. |
| 巻・号 | 20(12) |
| ページ | 671-7 |
| 公開日 | 2013-12-1 |
| DOI | 10.1038/cgt.2013.67 |
| PII | cgt201367 |
| PMID | 24201868 |
| PMC | PMC8185610 |
| MeSH | Animals Cell Line, Tumor Cytosine Deaminase / genetics Cytosine Deaminase / metabolism Disease Models, Animal Female Gene Expression Gene Order Genetic Therapy Genetic Vectors / administration & dosage Genetic Vectors / genetics* Humans Leukemia Virus, Gibbon Ape / genetics* Lung Neoplasms / genetics* Lung Neoplasms / metabolism Mesothelioma / genetics* Mesothelioma / metabolism Mesothelioma, Malignant Mice Prodrugs Transduction, Genetic* Virus Replication Xenograft Model Antitumor Assays |
| IF | 4.534 |
| 引用数 | 10 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) |