論文 - 詳細
| RRC ID | 44756 |
|---|---|
| 著者 | Kimura R, Kasamatsu A, Koyama T, Fukumoto C, Kouzu Y, Higo M, Endo-Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H, Uzawa K. |
| タイトル | Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by β-catenin translocation and MMP7 activation. |
| ジャーナル | BMC Cancer |
| Abstract |
BACKGROUND:Glutamate decarboxylase 1 (GAD1), a rate-limiting enzyme in the production of γ-aminobutyric acid (GABA), is found in the GABAergic neurons of the central nervous system. Little is known about the relevance of GAD1 to oral squamous cell carcinoma (OSCC). We investigated the expression status of GAD1 and its functional mechanisms in OSCCs. METHODS:We evaluated GAD1 mRNA and protein expressions in OSCC-derived cells using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunoblotting analyses. To assess the critical functions of GAD1, i.e., cellular proliferation, invasiveness, and migration, OSCC-derived cells were treated with the shRNA and specific GAD1 inhibitor, 3-mercaptopropionic acid (3-MPA). GAD1 expression in 80 patients with primary OSCCs was analyzed and compared to the clinicopathological behaviors of OSCC. RESULTS:qRT-PCR and immunoblotting analyses detected frequent up-regulation of GAD1 in OSCC-derived cells compared to human normal oral keratinocytes. Suppression of nuclear localization of β-catenin and MMP7 secretion was observed in GAD1 knockdown and 3-MPA-treated cells. We also found low cellular invasiveness and migratory abilities in GAD1 knockdown and 3-MPA-treated cells. In the clinical samples, GAD1 expression in the primary OSCCs was significantly (P < 0.05) higher than in normal counterparts and was correlated significantly (P < 0.05) with regional lymph node metastasis. CONCLUSIONS:Our data showed that up-regulation of GAD1 was a characteristic event in OSCCs and that GAD1 was correlated with cellular invasiveness and migration by regulating β-catenin translocation and MMP7 activation. GAD1 might play an important role in controlling tumoral invasiveness and metastasis in oral cancer. |
| 巻・号 | 13 |
| ページ | 555 |
| 公開日 | 2013-11-21 |
| DOI | 10.1186/1471-2407-13-555 |
| PII | 1471-2407-13-555 |
| PMID | 24261884 |
| PMC | PMC3866561 |
| MeSH | 3-Mercaptopropionic Acid / pharmacology Adult Aged Aged, 80 and over Carcinoma, Squamous Cell / enzymology* Carcinoma, Squamous Cell / secondary Cell Line, Tumor Cell Movement Cell Nucleus / metabolism Cell Proliferation Enzyme Activation Glutamate Decarboxylase / antagonists & inhibitors Glutamate Decarboxylase / physiology* Humans Matrix Metalloproteinase 7 / metabolism* Middle Aged Mouth Neoplasms / enzymology* Mouth Neoplasms / pathology Neoplasm Invasiveness Protein Transport beta Catenin / metabolism* |
| IF | 3.15 |
| 引用数 | 19 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Sa3(RCB0980) HO-1-u-1(RCB2102) Ca9-22(RCB1976) HSC-2(RCB1945) HSC-3(RCB1975) |