論文 - 詳細
| RRC ID | 44770 |
|---|---|
| 著者 | Qiu YH, Wei YP, Shen NJ, Wang ZC, Kan T, Yu WL, Yi B, Zhang YJ. |
| タイトル | miR-204 inhibits epithelial to mesenchymal transition by targeting slug in intrahepatic cholangiocarcinoma cells. |
| ジャーナル | Cell Physiol Biochem |
| Abstract |
BACKGROUND/AIMS:MicroRNAs (miRNAs) play critical roles during carcinogenesis and cancer progression. Down-regulation of miR-204 has been frequently observed in various cancers. In this study, we investigated the roles and mechanisms of miR-204 in human intrahepatic cholangiocarcinoma (ICC). METHODS:The relative expression of miR-204 in ICC tissues and cell lines was monitored by qRT-PCR. Effects of miR-204 were studied in human ICC cell lines HuH28 and HuCCT1, and cells were analyzed for proliferation, migration and invasion. Expression levels of miR-204 target gene Slug and EMT markers (E-cadherin and vimentin) in ICC cell lines and tissues were measured by qRT-PCR, western blotting and immunofluorescence. RESULTS:miR-204 was frequently downregulated in human ICC, and the low-level expression of miR-204 was significantly associated with lymph node metastasis. Overexpression of miR-204 dramatically suppressed ICC cell migration and invasion, as well as the epithelial-mesenchymal transition process (EMT). Slug was identified as a direct target of miR-204, and its downregulation by miR-204 in HuH28 cells reversed EMT, as shown by the increased expression of the epithelial marker E-cadherin and decreased expression of the mesenchymal marker vimentin. CONCLUSION:These findings suggest that miR-204 plays negative roles in the invasive and/or metastatic potential of ICC, and that its suppressive effects are mediated by repressing Slug expression. |
| 巻・号 | 32(5) |
| ページ | 1331-41 |
| 公開日 | 2013-1-1 |
| DOI | 10.1159/000354531 |
| PII | 000354531 |
| PMID | 24280681 |
| MeSH | Bile Duct Neoplasms Bile Ducts, Intrahepatic Cadherins / genetics Cell Line, Tumor Cell Movement / genetics Cell Proliferation Cholangiocarcinoma / genetics* Cholangiocarcinoma / pathology* Down-Regulation Epithelial-Mesenchymal Transition / genetics* Gene Expression Regulation, Neoplastic Humans Liver Neoplasms / genetics* Liver Neoplasms / pathology* Male MicroRNAs / metabolism* Middle Aged Snail Family Transcription Factors Transcription Factors / genetics* Vimentin / genetics |
| IF | 5.5 |
| 引用数 | 82 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |