Reference - Detail
|Author||Qiu YH, Wei YP, Shen NJ, Wang ZC, Kan T, Yu WL, Yi B, Zhang YJ.|
|Title||miR-204 inhibits epithelial to mesenchymal transition by targeting slug in intrahepatic cholangiocarcinoma cells.|
|Journal||Cell. Physiol. Biochem.|
BACKGROUND/AIMS:MicroRNAs (miRNAs) play critical roles during carcinogenesis and cancer progression. Down-regulation of miR-204 has been frequently observed in various cancers. In this study, we investigated the roles and mechanisms of miR-204 in human intrahepatic cholangiocarcinoma (ICC).
METHODS:The relative expression of miR-204 in ICC tissues and cell lines was monitored by qRT-PCR. Effects of miR-204 were studied in human ICC cell lines HuH28 and HuCCT1, and cells were analyzed for proliferation, migration and invasion. Expression levels of miR-204 target gene Slug and EMT markers (E-cadherin and vimentin) in ICC cell lines and tissues were measured by qRT-PCR, western blotting and immunofluorescence.
RESULTS:miR-204 was frequently downregulated in human ICC, and the low-level expression of miR-204 was significantly associated with lymph node metastasis. Overexpression of miR-204 dramatically suppressed ICC cell migration and invasion, as well as the epithelial-mesenchymal transition process (EMT). Slug was identified as a direct target of miR-204, and its downregulation by miR-204 in HuH28 cells reversed EMT, as shown by the increased expression of the epithelial marker E-cadherin and decreased expression of the mesenchymal marker vimentin.
CONCLUSION:These findings suggest that miR-204 plays negative roles in the invasive and/or metastatic potential of ICC, and that its suppressive effects are mediated by repressing Slug expression.
|MeSH||Bile Duct Neoplasms Bile Ducts, Intrahepatic Cadherins / genetics Cell Line, Tumor Cell Movement / genetics Cell Proliferation Cholangiocarcinoma / genetics* Cholangiocarcinoma / pathology* Down-Regulation Epithelial-Mesenchymal Transition / genetics* Gene Expression Regulation, Neoplastic Humans Liver Neoplasms / genetics* Liver Neoplasms / pathology* Male MicroRNAs / metabolism* Middle Aged Snail Family Transcription Factors Transcription Factors / genetics* Vimentin / genetics|
|WOS Category||PHYSIOLOGY CELL BIOLOGY|
|Human and Animal Cells|