論文 - 詳細
| RRC ID | 44798 |
|---|---|
| 著者 | Taguchi A, Kaku Y, Ohmori T, Sharmin S, Ogawa M, Sasaki H, Nishinakamura R. |
| タイトル | Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells. |
| ジャーナル | Cell Stem Cell |
| Abstract |
Recapitulating three-dimensional (3D) structures of complex organs, such as the kidney, from pluripotent stem cells (PSCs) is a major challenge. Here, we define the developmental origins of the metanephric mesenchyme (MM), which generates most kidney components. Unexpectedly, we find that posteriorly located T(+) MM precursors are developmentally distinct from Osr1(+) ureteric bud progenitors during the postgastrulation stage, and we identify phasic Wnt stimulation and stage-specific growth factor addition as molecular cues that promote their development into the MM. We then use this information to derive MM from PSCs. These progenitors reconstitute the 3D structures of the kidney in vitro, including glomeruli with podocytes and renal tubules with proximal and distal regions and clear lumina. Furthermore, the glomeruli are efficiently vascularized upon transplantation. Thus, by reevaluating the developmental origins of metanephric progenitors, we have provided key insights into kidney specification in vivo and taken important steps toward kidney organogenesis in vitro. |
| 巻・号 | 14(1) |
| ページ | 53-67 |
| 公開日 | 2014-1-2 |
| DOI | 10.1016/j.stem.2013.11.010 |
| PII | S1934-5909(13)00501-8 |
| PMID | 24332837 |
| MeSH | Animals Biomarkers / metabolism Cell Differentiation* Cells, Cultured Colony-Forming Units Assay Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Humans Immunoblotting Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Kidney / chemistry Kidney / cytology* Kidney / metabolism Mesoderm / cytology* Mesoderm / metabolism Nephrons / cytology* Nephrons / metabolism Organ Culture Techniques Organogenesis / physiology* Signal Transduction |
| IF | 20.86 |
| 引用数 | 325 |
| WOS 分野 | CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 114 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |