論文 - 詳細
| RRC ID | 44810 |
|---|---|
| 著者 | Hiyama T, Ozeki N, Mogi M, Yamaguchi H, Kawai R, Nakata K, Kondo A, Nakamura H. |
| タイトル | Matrix metalloproteinase-3 in odontoblastic cells derived from ips cells: unique proliferation response as odontoblastic cells derived from ES cells. |
| ジャーナル | PLoS One |
| Abstract |
We previously reported that matrix metalloproteinase (MMP)-3 accelerates wound healing following dental pulp injury. In addition, we reported that a proinflammatory cytokine mixture (tumor necrosis factor-α, interleukin (IL)-1β and interferon-γ) induced MMP-3 activity in odontoblast-like cells derived from mouse embryonic stem (ES) cells, suggesting that MMP-3 plays a potential unique physiological role in wound healing and regeneration of dental pulp in odontoblast-like cells. In this study, we tested the hypothesis that upregulation of MMP-3 activity by IL-1β promotes proliferation and apoptosis of purified odontoblast-like cells derived from induced pluripotent stem (iPS) and ES cells. Each odontoblast-like cell was isolated and incubated with different concentrations of IL-1β. MMP-3 mRNA and protein expression were assessed using RT-PCR and western blotting, respectively. MMP-3 activity was measured using immunoprecipitation and a fluorescence substrate. Cell proliferation and apoptosis were determined using ELISA for BrdU and DNA fragmentation, respectively. siRNA was used to reduce MMP-3 transcripts in these cells. Treatment with IL-1β increased MMP-3 mRNA and protein levels, and MMP-3 activity in odontoblast-like cells. Cell proliferation was found to markedly increase with no changes in apoptosis. Endogenous tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 were constitutively expressed during all experiments. The exocytosis inhibitor, Exo1, potently suppressed the appearance of MMP-3 in the conditioned medium. Treatment with siRNA against MMP-3 suppressed an IL-1β-induced increase in MMP-3 expression and activity, and also suppressed cell proliferation, but unexpectedly increased apoptosis in these cells (P<0.05). Exogenous MMP-3 was found to induce cell proliferation in odontoblast-like cells derived from iPS cells and ES cells. This siRNA-mediated increase in apoptosis could be reversed with exogenous MMP-3 stimulation (P<0.05). Taken together, IL-1β induced MMP-3-regulated cell proliferation and suppressed apoptosis in odontoblast-like cells derived from iPS and ES cells. |
| 巻・号 | 8(12) |
| ページ | e83563 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0083563 |
| PII | PONE-D-13-41129 |
| PMID | 24358294 |
| PMC | PMC3865184 |
| MeSH | Animals Cell Differentiation / drug effects Cell Differentiation / genetics Cell Proliferation* / drug effects Cells, Cultured Culture Media, Conditioned / pharmacology Embryonic Stem Cells / drug effects Embryonic Stem Cells / physiology* Gene Expression / drug effects Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / physiology* Interleukin-1beta / pharmacology Matrix Metalloproteinase 3 / genetics* Matrix Metalloproteinase 3 / metabolism Mice Mice, Inbred C57BL Odontoblasts / drug effects Odontoblasts / physiology* |
| IF | 2.74 |
| 引用数 | 18 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B6G-2(AES0003) |