RRC ID 44828
Author Yazaki Y, Oyane A, Tsurushima H, Araki H, Sogo Y, Ito A, Yamazaki A.
Title Coprecipitation of DNA-lipid complexes with apatite and comparison with superficial adsorption for gene transfer applications.
Journal J Biomater Appl
Abstract Apatite can mediate gene transfer into cells by serving as a safe and biocompatible immobilization matrix for DNA and transfection reagents. Recently, an apatite layer that immobilized DNA-lipid complexes was prepared by a coprecipitation process in a supersaturated calcium phosphate solution. This composite layer (DNA-lipid-apatite layer) showed a higher gene transfer capability than an apatite layer with superficially adsorbed DNA-lipid complexes (DNA-lipid-adsorbed apatite layer). In this study, the DNA-lipid-apatite layer and the DNA-lipid-adsorbed apatite layer were compared for their physicochemical properties and gene transfer capabilities. The higher gene transfer capability of the DNA-lipid-apatite layer compared with that of the DNA-lipid-adsorbed apatite layer was reconfirmed by a luciferase assay using epithelial-like CHO-K1 cells. Physicochemical structure analyses showed that the DNA-lipid-apatite layer possessed a larger capacity for DNA-lipid complexes than the DNA-lipid-adsorbed apatite layer. The DNA-lipid-apatite layer released DNA-lipid complexes in a slow and sustained manner, whereas the DNA-lipid-adsorbed apatite layer released them in short bursts. Consequently, the release of DNA-lipid complexes from the DNA-lipid-apatite layer was larger in amount and longer in duration than release from the DNA-lipid-adsorbed apatite layer. This difference in release profiles may be responsible for the higher gene transfer capability of the DNA-lipid-apatite layer compared with that of the DNA-lipid-adsorbed apatite layer. The coprecipitation process and the resulting DNA-lipid-apatite layer have many applications in tissue engineering.
Volume 28(6)
Pages 937-45
Published 2014-2-1
DOI 10.1177/0885328213486706
PII 28/6/937
PMID 24381202
MeSH Adsorption Animals Apatites / chemistry* CHO Cells Cricetinae Cricetulus DNA / chemistry* Gene Transfer Techniques* Lipids / chemistry* Microscopy, Electron, Scanning X-Ray Diffraction
IF 2.442
Times Cited 5
WOS Category ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
Resource
Human and Animal Cells