論文 - 詳細
| RRC ID | 44832 |
|---|---|
| 著者 | Tsuji S, Yamashita M, Kageyama T, Ohtsu T, Suzuki K, Kato S, Akitomi J, Furuichi M, Waga I. |
| タイトル | Hishot display--a new combinatorial display for obtaining target-recognizing peptides. |
| ジャーナル | PLoS One |
| Abstract |
Display technologies are procedures used for isolating target-recognizing peptides without using immunized animals. In this study, we describe a new display method, named Hishot display, that uses Escherichia coli and an expression plasmid to isolate target-recognizing peptides. This display method is based on the formation, in bacteria, of complexes between a polyhistidine (His)-tagged peptide including random sequences and the peptide-encoding mRNA including an RNA aptamer against the His-tag. When this system was tested using a sequence encoding His-tagged green fluorescent protein that included an RNA aptamer against the His-tag, the collection of mRNA encoding the protein was dependent on the RNA aptamer. Using this display method and a synthetic library of surrogate single-chain variable fragments consisting of VpreB and Ig heavy-chain variable domains, it was possible to isolate clones that could specifically recognize a particular target (intelectin-1 or tumor necrosis factor-α). These clones were obtained as soluble proteins produced by E. coli, and the purified peptide clones recognizing intelectin-1 could be used as detectors for sandwich enzyme-linked immunosorbent assays. The Hishot display will be a useful method to add to the repertoire of display technologies. |
| 巻・号 | 8(12) |
| ページ | e83108 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0083108 |
| PII | PONE-D-13-33067 |
| PMID | 24386149 |
| PMC | PMC3873924 |
| MeSH | Amino Acid Sequence Base Sequence Cell Surface Display Techniques / methods* Enzyme-Linked Immunosorbent Assay / methods* Escherichia coli / genetics* Escherichia coli / metabolism Genetic Vectors Green Fluorescent Proteins / analysis Molecular Sequence Data Peptide Library |
| IF | 2.74 |
| 引用数 | 1 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RK13(RCB0183) |