RRC ID 44836
著者 Uto S, Nishizawa S, Takasawa Y, Asawa Y, Fujihara Y, Takato T, Hoshi K.
タイトル Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model.
ジャーナル Biomed Res
Abstract The establishment of cartilage regenerative medicine has been an important issue in the clinical field, because cartilage has the poor ability of self-repair. Currently, tissue engineering using autologous chondrocytes has risen, but we should investigate more appropriate cell sources that can be obtained without any quantitative limitation. In this study, we focused on induced pluripotent stem (iPS) cells, in which the ethical hurdle does not seem higher than that of embryonic stem cells. Mouse iPS cells were transplanted into the mouse joint defect model of the knee. Strains of the transplants and hosts were arranged to be either closest (homology 75% in genetic background) or identical (100%). For transplantation, we embedded the iPS cells within the collagen hydrogel in order to obtain the effective administration of the cells into defects, which induced the differentiation of the iPS cells. At 8 weeks of transplantation, although the iPS cells with a 75% homology to the host in the genetic background tended to form teratoma, those of 100% showed a joint regeneration. GFP immunohistochemistry proved that the transplanted iPS cells were responsible for the bone and cartilage repair. Taking these results together, the iPS cells are regarded as a promising cell source for the cartilage tissue engineering.
巻・号 34(6)
ページ 281-8
公開日 2013-1-1
DOI 10.2220/biomedres.34.281
PII DN/JST.JSTAGE/biomedres/34.281
PMID 24389404
MeSH Animals Biomarkers / metabolism Bone Regeneration / physiology* Cartilage / pathology* Cell Differentiation Chondrocytes / cytology Chondrocytes / metabolism Collagen / chemistry Gene Expression Genes, Reporter Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Histocompatibility Testing Hydrogels Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / physiology Induced Pluripotent Stem Cells / transplantation* Knee Injuries / metabolism Knee Injuries / pathology Knee Injuries / therapy* Knee Joint / pathology* Mice Mice, Inbred DBA Mice, Transgenic Teratoma / pathology Wound Healing / physiology*
IF 1.429
引用数 21
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)