RRC ID 44853
著者 Maeda R, Ishii G, Neri S, Aoyagi K, Haga H, Sasaki H, Nagai K, Ochiai A.
タイトル Circulating CD14+CD204+ cells predict postoperative recurrence in non-small-cell lung cancer patients.
ジャーナル J Thorac Oncol
Abstract BACKGROUND:The expression of CD204 on macrophages in the stroma of the primary tumor is reportedly correlated with an unfavorable prognosis for lung cancer. The purpose of this study is to investigate the correlation among the number of CD204 tumor-associated macrophages infiltrating the stroma of the primary tumor, the number of circulating CD14CD204 cells from the pulmonary vein (PV), and recurrence-free probability in non-small-cell lung cancer patients.
METHODS:Human mononuclear cells were isolated from the PV of resected lungs. We examined the expressions of CD14 and CD204 on these cells by flow cytometry. Immunohistochemical staining for CD204 was performed in the resected specimens.
RESULTS:The number of CD14CD204 cells from the PV was found to be correlated with the number of CD204 tumor-associated macrophages identified in the stroma of the tumor. Significantly more cases with high levels of CD14CD204 cells from the PV were found to have developed early recurrences. CD14CD204 cells, which were polarized to the tumor-promoting phenotype cultured in lung cancer cell line-conditioned medium, facilitated the lung metastasis of cancer cells more effectively than CD14CD204 cells in our in vivo mouse model. In multivariate analysis, only the high number of CD14CD204 cells from the PV was found to be a statistically significant independent risk factor for early recurrence.
CONCLUSION:Our results showed the possibility that circulating CD14CD204 cells contribute to the metastasis of cancer cells. The blockage of circulating CD14CD204 cells activity may prevent postoperative recurrence in resected non-small-cell lung cancer patients.
巻・号 9(2)
ページ 179-88
公開日 2014-2-1
DOI 10.1097/JTO.0000000000000044
PII S1556-0864(15)30187-8
PMID 24419414
MeSH Adenocarcinoma / blood Adenocarcinoma / secondary* Adenocarcinoma / surgery Animals Biomarkers, Tumor / blood* Carcinoma, Non-Small-Cell Lung / blood Carcinoma, Non-Small-Cell Lung / secondary* Carcinoma, Non-Small-Cell Lung / surgery Female Flow Cytometry Humans Immunoenzyme Techniques Lipopolysaccharide Receptors / blood* Lung Neoplasms / blood Lung Neoplasms / pathology* Lung Neoplasms / surgery Macrophages / metabolism Macrophages / pathology Male Mice Middle Aged Neoplasm Recurrence, Local / diagnosis* Neoplasm Staging Postoperative Period Prognosis Scavenger Receptors, Class A / blood* Stromal Cells / metabolism Stromal Cells / pathology
IF 13.357
引用数 15
WOS 分野 RESPIRATORY SYSTEM ONCOLOGY
リソース情報
ヒト・動物細胞 A549