Reference - Detail
| RRC ID | 44872 |
|---|---|
| Author | Kobayashi E, Satow R, Ono M, Masuda M, Honda K, Sakuma T, Kawai A, Morioka H, Toyama Y, Yamada T. |
| Title | MicroRNA expression and functional profiles of osteosarcoma. |
| Journal | Oncology |
| Abstract |
OBJECTIVE:Osteosarcoma (OS) is the most frequent primary malignant bone tumor in children and young adults. Although the introduction of combined neoadjuvant chemotherapy has significantly prolonged survival, the outcome for OS patients showing a poor response to chemotherapy is still unfavorable. In order to develop new therapeutic approaches, elucidation of the entire molecular pathway regulating OS cell proliferation would be desirable. METHODS:MicroRNA (miRNA) are highly conserved noncoding RNA that play important roles in the development and progression of various other cancers. Using miRNA microarrays capable of detecting a known number of 933 miRNA, 108 miRNA were found to be commonly expressed in 24 samples of OS tissue and subjected to a cell proliferation assay. RESULTS:We found that inhibition of 5 let-7 family miRNA (hsa-let-7a, b, f, g and i) significantly suppressed the proliferation of OS cells. Using a quantitative shotgun proteomics approach, we also found that the let-7 family miRNA regulated the expression of vimentin and serpin H1 proteins. CONCLUSIONS:Our present results indicate the involvement of let-7 family miRNA in regulation of the cell proliferation as well as epithelial-mesenchymal transition of OS. Thus, let-7 family miRNA may potentially provide novel targets for the development of therapeutic strategies against OS. |
| Volume | 86(2) |
| Pages | 94-103 |
| Published | 2014-1-1 |
| DOI | 10.1159/000357408 |
| PII | 000357408 |
| PMID | 24457375 |
| MeSH | Adolescent Adult Amino Acid Sequence Bone Neoplasms / genetics Bone Neoplasms / metabolism* Cell Line, Tumor Cell Proliferation Child Female Gene Expression Regulation, Neoplastic HSP47 Heat-Shock Proteins / chemistry HSP47 Heat-Shock Proteins / genetics HSP47 Heat-Shock Proteins / metabolism Humans Male MicroRNAs / genetics MicroRNAs / metabolism* Molecular Sequence Data Osteosarcoma / genetics Osteosarcoma / metabolism* RNA Interference Transcriptome Vimentin / chemistry Vimentin / genetics Vimentin / metabolism Young Adult |
| IF | 2.642 |
| Times Cited | 24 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | NOS-1(RCB1032) HuO-3N1(RCB2104) HuO 9N2 (O9N2)(RCB2532) |