RRC ID 44877
Author Lee JS, Hong JM, Jung JW, Shim JH, Oh JH, Cho DW.
Title 3D printing of composite tissue with complex shape applied to ear regeneration.
Journal Biofabrication
Abstract In the ear reconstruction field, tissue engineering enabling the regeneration of the ear's own tissue has been considered to be a promising technology. However, the ear is known to be difficult to regenerate using traditional methods due to its complex shape and composition. In this study, we used three-dimensional (3D) printing technology including a sacrificial layer process to regenerate both the auricular cartilage and fat tissue. The main part was printed with poly-caprolactone (PCL) and cell-laden hydrogel. At the same time, poly-ethylene-glycol (PEG) was also deposited as a sacrificial layer to support the main structure. After complete fabrication, PEG can be easily removed in aqueous solutions, and the procedure for removing PEG has no effect on the cell viability. For fabricating composite tissue, chondrocytes and adipocytes differentiated from adipose-derived stromal cells were encapsulated in hydrogel to dispense into the cartilage and fat regions, respectively, of ear-shaped structures. Finally, we fabricated the composite structure for feasibility testing, satisfying expectations for both the geometry and anatomy of the native ear. We also carried out in vitro assays for evaluating the chondrogenesis and adipogenesis of the cell-printed structure. As a result, the possibility of ear regeneration using 3D printing technology which allowed tissue formation from the separately printed chondrocytes and adipocytes was demonstrated.
Volume 6(2)
Pages 024103
Published 2014-6
DOI 10.1088/1758-5082/6/2/024103
PMID 24464765
MeSH Adipogenesis / physiology Adipose Tissue / cytology Biocompatible Materials Bioprinting / methods* Cells, Cultured Chondrogenesis / physiology Ear / physiology* Humans Hydrogels Polyethylene Glycols Printing, Three-Dimensional* Regeneration / physiology* Stem Cells / cytology Tissue Engineering / methods* Tissue Scaffolds*
IF 7.236
Times Cited 176
Human and Animal Cells