論文 - 詳細
| RRC ID | 44879 |
|---|---|
| 著者 | Hagiwara M, Kokubu E, Sugiura S, Komatsu T, Tada H, Isoda R, Tanigawa N, Kato Y, Ishida N, Kobayashi K, Nakashima M, Ishihara K, Matsushita K. |
| タイトル | Vinculin and Rab5 complex is required [correction of requited]for uptake of Staphylococcus aureus and interleukin-6 expression. |
| ジャーナル | PLoS One |
| Abstract |
Vinculin, a 116-kDa membrane cytoskeletal protein, is an important molecule for cell adhesion; however, little is known about its other cellular functions. Here, we demonstrated that vinculin binds to Rab5 and is required for Staphylococcus aureus (S. aureus) uptake in cells. Viunculin directly bound to Rab5 and enhanced the activation of S. aureus uptake. Over-expression of active vinculin mutants enhanced S. aureus uptake, whereas over-expression of an inactive vinculin mutant decreased S. aureus uptake. Vinculin bound to Rab5 at the N-terminal region (1-258) of vinculin. Vinculin and Rab5 were involved in the S. aureus-induced phosphorylation of MAP kinases (p38, Erk, and JNK) and IL-6 expression. Finally, vinculin and Rab5 knockdown reduced infection of S. aureus, phosphorylation of MAPKs and IL-6 expression in murine lungs. Our results suggest that vinculin binds to Rab5 and that these two molecules cooperatively enhance bacterial infection and the inflammatory response. |
| 巻・号 | 9(1) |
| ページ | e87373 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0087373 |
| PII | PONE-D-13-46228 |
| PMID | 24466349 |
| PMC | PMC3900708 |
| MeSH | Albumins / metabolism Animals Bacterial Adhesion / physiology* COS Cells Chlorocebus aethiops Escherichia coli Female Gene Knockdown Techniques Genetic Vectors / genetics HeLa Cells Humans Immunoprecipitation Interleukin-6 / metabolism* Isoquinolines / metabolism Lung / metabolism Lung / microbiology Mice Mice, Inbred BALB C Staphylococcus aureus / physiology* Transferrin / metabolism Vinculin / metabolism* rab5 GTP-Binding Proteins / metabolism* |
| IF | 2.74 |
| 引用数 | 14 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) HeLa |